Linked Life Data

8011678

Source:http://linkedlifedata.com/resource/pubmed/id/8011678

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-7-25
pubmed:abstractText
The simultaneous incorporation of a saturated fatty acid in the sn-1 position and an unsaturated fatty acid in the sn-2 position in phosphatidylcholine (PC) and ethanolamine (PE) was studied in isolated liver cells. We combined a saturated fatty acid, 16:0 or 18:0 and an unsaturated fatty acid substrate, 18:2,n-6 or 20:4,n-6. In this situation the saturated fatty acids were preferentially oxidized and the unsaturated fatty acids were preferentially esterified in PL and TG. Addition of unlabelled 16:0 increased the incorporation of [14C]18:2 in 16:0-18:2 in PC and PE, reduced the incorporation in 18:2-18:2 but did not reduce the incorporation in 18:0-18:2. 18:0 increased the esterification of [14C]18:2 in 18:0-18:2, reduced the incorporation in 18:2-18:2 but did not reduce the incorporation in 16:0-18:2. The latter is the dominating 14C-labelled species formed from [14C]18:2 also in the presence of unlabelled 18:0. Addition of 20:4 stimulated the incorporation of [14C]16:0 in 16:0-20:4 and markedly reduced the formation of 16:0-18:2, 16:0-18:1 and 16:0-22:6. Addition of 18:2 increased the incorporation of [14C]16:0 in 16:0-18:2 and reduced the formation of 16:0-20:4 and 16:0-18:1. It is concluded that the unsaturated fatty acids 18:2 or 20:4 have a stronger impact on the synthesis of phospholipid molecular species than the saturated fatty acids 16:0 or 18:0 have. Thus 20:4,n-6 and 18:2,n-6 are able to direct available [14C]16:0 or [14C]18:0 to the sn-1 position. 16:0 and 18:0 are not in the same way able to direct [14C]18:2,n-6 to the synthesis of 16:0-18:2 or 18:0-18:2 at the expense of other 14C-labelled molecular species.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
1213
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
39-45
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Biosynthesis of phospholipid molecular species in isolated liver cells studied by combining fatty acid substrates esterified in the sn-1 and sn-2 positions.
pubmed:affiliation
Institute of Clinical Biochemistry, University of Oslo, Rikshospitalet, Norway.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't