Linked Life Data

8010060

Source:http://linkedlifedata.com/resource/pubmed/id/8010060

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1994-7-20
pubmed:abstractText
Oridonin (Ori) is an active principle isolated from Rabdosia rubescens. The cytotoxic effect of the combination of Ori and cisplatin was tested by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay. IC50 of cisplatin to cultured S180 cells in 24 h was 9.38 micrograms.ml-1. When the cells were treated with cisplatin plus Ori 0.5 and 1 microgram.ml-1, the IC50 were 1/3.4 and 1/6.7, respectively, of that with cisplatin alone. Modified alkaline elution was used to detect the DNA interstrand cross-link and DNA-protein cross-link in S180 cells induced by the 2 drugs. A greater amount of DNA cross-link was detected when the cells were treated with cisplatin plus Ori than with cisplatin alone (P < 0.05). After lysis by proteinase K, a reduction in DNA cross-link was seen, which suggested that the drugs could produce both kinds of DNA cross-link.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0253-9756
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
561-4
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Synergistic effect of oridonin and cisplatin on cytotoxicity and DNA cross-link against mouse sarcoma S180 cells in culture.
pubmed:affiliation
Department of Pharmacology, He-nan Institute of Medical Sciences, Zhengzhou, China.
pubmed:publicationType
Journal Article