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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1994-7-19
|
| pubmed:abstractText |
A p100-GAP isoform, generated by an alternative splicing mechanism that eliminates the 180 hydrophobic amino acids at the amino terminus of p120-GAP, has been described in human placenta, in addition to the known p120GAP and neurofibromin. This p100-GAP possesses full Ras-GTPase stimulating activity. p120-GAP is ubiquitously localized in the cytosol while the localization of p100-GAP is unknown. Here we have explored the precise localization of p100-GAP and show that p100-GAP is present only in extracts of primate placenta. It is abundant in both human and Maccaca Rhesus placentae, where it is present in far larger amounts than p120-GAP. The p100-GAP is species-specific since it was not detected in the placenta of pig, sheep, mouse or rat. p100-GAP was also found to be organ-specific, since it was not detectable in organs other than the placenta. In this connection, we substantiated our previous finding that p100-GAP is mainly localized in the trophoblasts. Both subcellular trophoblast fractionation and immunofluorescence analyses showed that this protein was distributed between the cytosol, plasma membrane and a fraction bound to the nucleus, but not inside it. This highly restrictive specificity of p100-GAP localization in relation to species, organ and cell type, confirms the extreme singularity of this protein, and strongly suggests a particular specific function in the trophoblast.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0021-9533
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
107 ( Pt 3)
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
427-35
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8006063-Amino Acid Sequence,
pubmed-meshheading:8006063-Animals,
pubmed-meshheading:8006063-Female,
pubmed-meshheading:8006063-Fluorescent Antibody Technique,
pubmed-meshheading:8006063-GTPase-Activating Proteins,
pubmed-meshheading:8006063-Humans,
pubmed-meshheading:8006063-Macaca fascicularis,
pubmed-meshheading:8006063-Macaca mulatta,
pubmed-meshheading:8006063-Mice,
pubmed-meshheading:8006063-Molecular Sequence Data,
pubmed-meshheading:8006063-Organ Specificity,
pubmed-meshheading:8006063-Placenta,
pubmed-meshheading:8006063-Pregnancy,
pubmed-meshheading:8006063-Proteins,
pubmed-meshheading:8006063-Rats,
pubmed-meshheading:8006063-Sheep,
pubmed-meshheading:8006063-Species Specificity,
pubmed-meshheading:8006063-Trophoblasts,
pubmed-meshheading:8006063-ras GTPase-Activating Proteins
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Species specificity and organ, cellular and subcellular localization of the 100 kDa Ras GTPase activating protein.
|
| pubmed:affiliation |
Unité 245 INSERM, Hôpital Saint-Antoine, Paris, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|