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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
25
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pubmed:dateCreated |
1994-7-20
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pubmed:abstractText |
The human and mouse cripto-1 (CR-1) genes can code for proteins related in structure to epidermal growth factor (EGF). A specific 36-kDa immunoreactive protein was detected by Western blot analysis in human cell lines that express CR-1 mRNA but not in cell lines that fail to express this transcript. Immunoprecipitation of GEO colon carcinoma or mouse embryonal carcinoma cells detected 27-29-kDa and 24-kDa proteins, respectively. Cell lysates and conditioned medium that were prepared from several CHO clones and were expressing either a recombinant human or mouse CR-1 cDNA contained immunospecific 27-29-kDa and 24-kDa proteins, respectively. Monensin or tunicamycin treatment resulted in a shift of the 27-29-kDa human CR-1 protein to 24 kDa and 20 kDa, respectively. The 20-kDa protein was also observed after digestion of the 27-29-kDa human CR-1 protein with N-glycosidase F. Using two CR-1 synthetic refolded peptides that correspond to the EGF-like domain of the human CR-1 sequence or conditioned medium obtained from human CR-1 expressing CHO cells, growth stimulatory activity could be detected on non-transformed human mammary epithelial cells and on two human breast cancer cell lines. EGF receptor-blocking antibody did not inhibit the growth stimulatory action of the CR-1 protein. Likewise, the CR-1 refolded peptides or conditioned medium from the human CR-1-expressing CHO cells failed to inhibit the binding of 125I-EGF in an EGF-radioreceptor assay. These data demonstrate that the CR-1 is a glycoprotein that can function as a growth factor through an EGF receptor-independent pathway.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/GPI-Linked Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TDGF1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tdgf1 protein, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
24
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pubmed:volume |
269
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
17320-8
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:8006041-Amino Acid Sequence,
pubmed-meshheading:8006041-Animals,
pubmed-meshheading:8006041-Blotting, Western,
pubmed-meshheading:8006041-CHO Cells,
pubmed-meshheading:8006041-Cell Division,
pubmed-meshheading:8006041-Cloning, Molecular,
pubmed-meshheading:8006041-Cricetinae,
pubmed-meshheading:8006041-DNA, Complementary,
pubmed-meshheading:8006041-Epidermal Growth Factor,
pubmed-meshheading:8006041-GPI-Linked Proteins,
pubmed-meshheading:8006041-Gene Expression,
pubmed-meshheading:8006041-Growth Substances,
pubmed-meshheading:8006041-Humans,
pubmed-meshheading:8006041-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:8006041-Membrane Glycoproteins,
pubmed-meshheading:8006041-Mice,
pubmed-meshheading:8006041-Molecular Sequence Data,
pubmed-meshheading:8006041-Molecular Weight,
pubmed-meshheading:8006041-Neoplasm Proteins,
pubmed-meshheading:8006041-Peptides,
pubmed-meshheading:8006041-Precipitin Tests,
pubmed-meshheading:8006041-RNA, Messenger,
pubmed-meshheading:8006041-Radioligand Assay,
pubmed-meshheading:8006041-Recombinant Proteins
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pubmed:year |
1994
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pubmed:articleTitle |
Identification and biological characterization of an epidermal growth factor-related protein: cripto-1.
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pubmed:affiliation |
Tumor Growth Factor Section, Laboratory of Tumor Immunology and Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892.
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pubmed:publicationType |
Journal Article
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