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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
24
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pubmed:dateCreated |
1995-1-23
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pubmed:abstractText |
We have isolated and characterized single-amino-acid substitution mutants of RNA polymerase alpha subunit defective in CAP-dependent transcription at the lac promoter but not defective in CAP-independent transcription. Our results establish that (1) amino acids 258-265 of alpha constitute an "activation target" essential for CAP-dependent transcription at the lac promoter but not essential for CAP-independent transcription, (2) amino acid 261 is the most critical amino acid of the activation target, (3) amino acid 261 is distinct from the determinants for alpha-DNA interaction, and (4) the activation target may fold as a surface amphipathic alpha-helix. We propose a model for transcriptional activation at the lac promoter that integrates these and other recent results regarding transcriptional activation and RNA polymerase structure and function.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0890-9369
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
8
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pubmed:geneSymbol |
lac,
rpoA
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3058-67
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8001824-Amino Acid Sequence,
pubmed-meshheading:8001824-Base Sequence,
pubmed-meshheading:8001824-Cyclic AMP Receptor Protein,
pubmed-meshheading:8001824-DNA, Bacterial,
pubmed-meshheading:8001824-DNA Primers,
pubmed-meshheading:8001824-DNA-Directed RNA Polymerases,
pubmed-meshheading:8001824-Escherichia coli,
pubmed-meshheading:8001824-Gene Expression Regulation, Bacterial,
pubmed-meshheading:8001824-Macromolecular Substances,
pubmed-meshheading:8001824-Molecular Sequence Data,
pubmed-meshheading:8001824-Mutagenesis,
pubmed-meshheading:8001824-Oligodeoxyribonucleotides,
pubmed-meshheading:8001824-Plasmids,
pubmed-meshheading:8001824-Point Mutation,
pubmed-meshheading:8001824-Promoter Regions, Genetic,
pubmed-meshheading:8001824-Transcription, Genetic
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pubmed:year |
1994
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pubmed:articleTitle |
Location, structure, and function of the target of a transcriptional activator protein.
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pubmed:affiliation |
Department of Chemistry, Rutgers University, New Brunswick, New Jersey 08855.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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