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pubmed-article:8000565pubmed:abstractTextThe voltage-gated currents of the astrocytes associated with the retinal capillaries of the rabbit retina were studied using whole-cell patch clamp recording. The resting potential of these cells was -70 +/- 4.8 mV (mean +/- SEM; n = 54), and the input resistance and cell capacitance were 558 +/- 3.6 M omega and 19.5 +/- 1.8 pF respectively. Depolarization to potentials positive to -50 mV evoked rapidly activating inward and outward currents. The inward current was transient, eliminated by substitution of choline for Na+ in the bathing solution, and reduced by 50% in the presence of 1 microM tetrodotoxin. The time-to-peak of the Na+ current was more than twice that for the Na+ current found in retinal neurons. The glial Na+ current was half-inactivated at -55 mV. A transient component of the outward K+ current was blocked by external 4-aminopyridine while a more sustained component was blocked by external tetraethylammonium. At potentials between -150 and -50 mV the membrane behaved Ohmically. Voltage-gated currents in retinal astrocytes recorded in situ appear qualitatively similar to those described for some glial cells in vitro.lld:pubmed
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pubmed-article:8000565pubmed:dateRevised2009-9-29lld:pubmed
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pubmed-article:8000565pubmed:articleTitleVoltage-gated currents in rabbit retinal astrocytes.lld:pubmed
pubmed-article:8000565pubmed:affiliationDepartment of Physiology, University College London, UK.lld:pubmed
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pubmed-article:8000565pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed