Linked Life Data

8000389

Source:http://linkedlifedata.com/resource/pubmed/id/8000389

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1995-1-26
pubmed:abstractText
A specific sandwich enzyme-linked immunosorbent assay (ELISA) was developed for human growth hormone (hGH) with molecular weight of 22 kDa (22k hGH). To prepare the 22k hGH specific antibody, a peptide (P-15) corresponding to residues 32-46 in 22k hGH was synthesized and used to immunize rabbits, because a 20k hGH variant was known to lack this peptide sequence. The assay method was developed using a combination of an anti P-15 polyclonal antibody (YK-2) coated on the microtiter well as a catching antibody and another polyclonal antibody against 22k hGH as a peroxidase-conjugated detecting antibody. The assay sensitivity was 1 ng/ml for 22k hGH, and cross-reactivity with other pituitary protein hormones as well as the 20k hGH variant was less than 0.01%, respectively. Furthermore, the 22k hGH content was measured precisely even in the presence of 20k hGH in this ELISA system.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0918-6158
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
977-9
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
A 22 kDa human growth hormone specific enzyme-linked immunosorbent assay.
pubmed:affiliation
Biochemistry Research Laboratories, JCR Pharmaceuticals Co., Ltd., Kobe, Japan.
pubmed:publicationType
Journal Article