Linked Life Data

7999795

Source:http://linkedlifedata.com/resource/pubmed/id/7999795

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-1-26
pubmed:databankReference
pubmed:abstractText
Hepatocyte nuclear factor 4 (HNF-4) is a liver-enriched transcription factor involved in the expression of many liver-specific genes. In the preceding communication (Hata, S., Tsukamoto, T. and Osumi, T. (1992) Biochim. Biophys. Acta 1131, 211-213), we reported the presence of two isoforms of mRNA for HNF-4 in rat liver and kidney. The longer isoform contained a segment of 30 bases which was not present in the shorter one. As an initial step to determine whether or not other mammals have these mRNA isoforms, we isolated a cDNA for mouse HNF-4 using the rat HNF-4 gene as a probe. The cDNA had an open reading frame for a 465 amino acid polypeptide. The deduced amino acid sequence was remarkably conserved between mouse HNF-4 and rat HNF-4 (99.6% identical). Moreover, like the cDNA for the larger rat isoform, the mouse cDNA contained an extra segment of 30 bp in the coding region near the C-terminus. Blotting analyses showed that the mRNA is about 3.7 kb in size and that a single copy of the gene is present in the mouse genome. Next we carried out the polymerase chain reaction (PCR) using primers located just upstream and downstream of the extra segment. Two PCR products were amplified from a mouse liver cDNA library. Determination of their nucleotide sequences proved that they exactly corresponded to the two rat isoforms. Finally, we amplified a DNA fragment (1.1 kb in size) from mouse genomic DNA using the same PCR primers as above. Its nucleotide sequence unequivocally confirmed that different splice donor sites were used to generate the two isoforms.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
1260
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
55-61
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:7999795-Amino Acid Sequence, pubmed-meshheading:7999795-Animals, pubmed-meshheading:7999795-Base Sequence, pubmed-meshheading:7999795-Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, pubmed-meshheading:7999795-Blotting, Northern, pubmed-meshheading:7999795-Blotting, Southern, pubmed-meshheading:7999795-DNA, Complementary, pubmed-meshheading:7999795-DNA-Binding Proteins, pubmed-meshheading:7999795-Hepatocyte Nuclear Factor 4, pubmed-meshheading:7999795-Liver, pubmed-meshheading:7999795-Mice, pubmed-meshheading:7999795-Mice, Inbred BALB C, pubmed-meshheading:7999795-Molecular Sequence Data, pubmed-meshheading:7999795-Phosphoproteins, pubmed-meshheading:7999795-RNA, Messenger, pubmed-meshheading:7999795-RNA Splicing, pubmed-meshheading:7999795-Transcription Factors
pubmed:year
1995
pubmed:articleTitle
Identification of two splice isoforms of mRNA for mouse hepatocyte nuclear factor 4 (HNF-4).
pubmed:affiliation
Department of Life Science, Faculty of Science, Himeji Institute of Technology, Hyogo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't