Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1977-7-29
pubmed:abstractText
A continuous cell line of human spleen has been obtained. The methods of seeding large numbers of cells and prolonged cultivation of the culture played an important role in isolation of this cell line; both methods have contributed to the creation of conditions for gradual reconstruction of the cellular metabolism in the absence of which the cells wound not be able to get adapted to contiuous cultivation. As the authors failed to obtain a contiuous cell line from a single cell colony the method of "shaking" was applied. The culture was obtained only in case of a 1% PHA solution was added to the nutrient medium.
pubmed:language
rus
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0365-9615
pubmed:author
pubmed:issnType
Print
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
995-8
pubmed:dateRevised
2008-10-8
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
[Isolation and characteristics of a continuous human splenic cell culture].
pubmed:publicationType
Journal Article, English Abstract