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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1995-1-17
|
| pubmed:abstractText |
We have developed a simple PCR based approach for synthesizing high-molecular-weight DNA multimers of short oligonucleotide elements in yields sufficient for preparing DNA affinity columns or for using as probes to detect sequence-specific DNA binding proteins. The technique is rapid, efficient and produces multimers consisting of 30-100 repeating units.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0006-291X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
30
|
| pubmed:volume |
205
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
475-81
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7999067-Base Sequence,
pubmed-meshheading:7999067-Biopolymers,
pubmed-meshheading:7999067-Chromatography, Affinity,
pubmed-meshheading:7999067-DNA,
pubmed-meshheading:7999067-Electrophoresis, Agar Gel,
pubmed-meshheading:7999067-Molecular Sequence Data,
pubmed-meshheading:7999067-Molecular Weight,
pubmed-meshheading:7999067-Polymerase Chain Reaction
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
A rapid and efficient PCR-based method for synthesizing high-molecular-weight multimers of oligonucleotides.
|
| pubmed:affiliation |
Laboratory of Structural Biology and Molecular Medicine, UCLA School of Medicine 90024.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|