Linked Life Data

7997188

Source:http://linkedlifedata.com/resource/pubmed/id/7997188

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1995-1-17
pubmed:abstractText
Recent outbreaks of human listeriosis have emphasized the importance of food in the etiology of epidemic listeriosis, suggesting that the gastrointestinal tract is the natural site of entry for Listeria monocytogenes into the organism. L. monocytogenes invasion of finite cell lines derived from the porcine ileum exhibited a 100-fold lower penetration level, without any intracellular multiplication, when compared to CaCo-2 cells, a widely used in vitro model for L. monocytogenes invasion. Same results were obtained with both pig kidney primary cells and mouse kidney finite cell lines. To demonstrate that cell immortalization enhances L. monocytogenes invasion, finite cell lines from porcine ileum and from murine kidney were immortalized by Simian virus 40 (SV40) large T oncogene. Unlike their untransformed counterparts, the immortal cells obtained were invaded by L. monocytogenes, as observed for CaCo-2 cells as well as for spontaneously immortal human (HeLa) and murine (3T3) cell lines. Extensive electron microscopy examinations of porcine epithelioid cells infected by L. monocytogenes showed numerous bacteria within the immortal cells, whereas neither intracellular bacteria nor any bacterial antigen were revealed inside finite cell lines. These data suggested that L. monocytogenes were not destroyed inside finite cell lines but only poorly entered the finite or primary cells. Speculating that L. monocytogenes invasion is under control of differentiation or proliferation of the cells, only an enterocyte subset at a defined state of differentiation or expressing particular receptors could be invaded in vivo.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0300-8584
pubmed:author
pubmed:issnType
Print
pubmed:volume
183
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
145-58
pubmed:dateRevised
2007-2-21
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Cell immortalization enhances Listeria monocytogenes invasion.
pubmed:affiliation
Centre de Recherche, Institut National de la Recherche Agronomique de Tours-Nouzilly, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't