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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
1995-1-13
|
| pubmed:abstractText |
The transport of monocarboxylic acid drugs such as salicylic acid was examined in the human colon adenocarcinoma cell line, Caco-2 cells that possess intestinal epithelia-like properties. [14C]Salicylic acid transport was pH-dependent and appeared to follow the pH-partition hypothesis. However, 10 mM unlabelled salicylic acid significantly reduced the permeability coefficient of [14C]salicylic acid. Kinetic analysis of the concentration dependence of the permeation rate of salicylic acid across Caco-2 cells showed both saturable (Kt = 5.28 +/- 0.72 mM Jmax = 36.6 +/- 3.54 nmol min-1 (mg protein)-1) and nonsaturable (kd = 0.37 +/- 0.08 microL min-1 (mg protein)-1) processes. The permeation rate of [14C]salicylic acid was competitively inhibited by both acetic acid and benzoic acid, which were demonstrated in our previous studies to be transported in the carrier-mediated-transport mechanism which is responsible for monocarboxylic acids. Furthermore, certain monocarboxylic acids significantly inhibited [14C]salicylic acid transport, whereas salicylamide and dicarboxylic acids such as succinic acid did not. From these results, it was concluded that the transcellular transport of [14C]salicylic acid across Caco-2 cells is by the pH-dependent and carrier-mediated transport mechanism specific for monocarboxylic acids.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Acetic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Arrhythmia Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Benzoates,
http://linkedlifedata.com/resource/pubmed/chemical/Benzoic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Salicylic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Salicylic Acids
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0022-3573
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
46
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
567-70
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7996384-Acetic Acid,
pubmed-meshheading:7996384-Acetic Acids,
pubmed-meshheading:7996384-Adenocarcinoma,
pubmed-meshheading:7996384-Anti-Arrhythmia Agents,
pubmed-meshheading:7996384-Benzoates,
pubmed-meshheading:7996384-Benzoic Acid,
pubmed-meshheading:7996384-Biological Transport, Active,
pubmed-meshheading:7996384-Cell Membrane Permeability,
pubmed-meshheading:7996384-Colonic Neoplasms,
pubmed-meshheading:7996384-Dose-Response Relationship, Drug,
pubmed-meshheading:7996384-Humans,
pubmed-meshheading:7996384-Hydrogen-Ion Concentration,
pubmed-meshheading:7996384-Intestinal Absorption,
pubmed-meshheading:7996384-Linear Models,
pubmed-meshheading:7996384-Salicylic Acid,
pubmed-meshheading:7996384-Salicylic Acids,
pubmed-meshheading:7996384-Temperature,
pubmed-meshheading:7996384-Tumor Cells, Cultured
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
pH-dependent and carrier-mediated transport of salicylic acid across Caco-2 cells.
|
| pubmed:affiliation |
Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Kanazawa University, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|