Linked Life Data

7996027

Source:http://linkedlifedata.com/resource/pubmed/id/7996027

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1995-1-13
pubmed:abstractText
Direct immunoperoxidase technique using human monoclonal antibody (C7) against a p65 antigen of cytomegalovirus (CMV) has been utilized to detect CMV antigen-positive leukocytes in the peripheral blood (CMV antigenemia). This technique was evaluated for enumeration of CMV antigen-positive leukocytes. The parameters included stability of the reagents, reproducibility of the results and quantitativity of the detection. The horseradish peroxidase (HRR)-labeled F(ab')2 fractions of C7 antibody were found to be superior to the equivalent Fab' fractions, because the former was much more stable than the latter. Enumeration of the CMV antigen-positive leukocytes were very reproducible and quantitative. The detection limit was one CMV antigen-positive cell per 50,000 leukocytes. Thus, this technique is reliable and practical to detect the CMV antigenemia, and it will contribute to early diagnosis of CMV diseases and initiation of antiviral therapy.
pubmed:language
jpn
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0387-5911
pubmed:author
pubmed:issnType
Print
pubmed:volume
68
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1278-84
pubmed:dateRevised
2008-12-16
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
[Evaluation of direct immunoperoxidase technique using F(ab')2 fractions of anti-cytomegalovirus human monoclonal antibody for enumeration of cytomegalovirus antigen-positive leukocytes].
pubmed:affiliation
Department of Microbiology, Miyazaki Medical College.
pubmed:publicationType
Journal Article, Comparative Study, English Abstract