Linked Life Data

7995653

Source:http://linkedlifedata.com/resource/pubmed/id/7995653

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1995-1-13
pubmed:abstractText
This study assessed the condition of spermatozoa from the proximal vas deferens of men after vasectomy. The fluids of both proximal vas deferens were collected from 67 vasectomized men by cannulating the vas deferens at the time of vasectomy reversal. Selected sperm parameters were analysed after incubation of the spermatozoa for 30 min at 37 degrees C. Sperm concentration in the proximal vas from vasectomized men (16,312 +/- 21,496 million per ml, geometric mean: 7948 +/- 398 million per ml) was significantly higher than that of fertile men and was maintained at a constant level independent of the duration of vas obstruction. The means of sperm motility (36.2 +/- 26.2%), spermatozoa with normal morphology (50.7 +/- 21.7%), sperm viability (53.0 +/- 25.3%) and hypo-osmotic swelling test (HOS-test, 53.9 +/- 21.7%) were statistically lower than the respective values for normal fertile men. There was no significant correlation between the duration of vas obstruction and the above semen parameters. In 46.4% of vas fluids all spermatozoa were immotile and this condition was more common after 3 years of vasectomy. Immotile spermatozoa in the proximal vas fluids at the time of vasectomy reversal may be an important factor for predicting semen quality and fertilizing ability after vasovasostomy. There were no significant differences in the results of sperm-cervical mucus penetration test (CMPT) between spermatozoa from vasectomized and fertile men. Antisperm antibodies on the surface of spermatozoa from the vas of vasectomized men were determined by the immunobead test (IBT; 78.6% for IgG, 32.1% for IgA) and sperm cervical mucus contact test (SCMC, 36.4%).(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:keyword
http://linkedlifedata.com/resource/pubmed/keyword/Asia, http://linkedlifedata.com/resource/pubmed/keyword/Biology, http://linkedlifedata.com/resource/pubmed/keyword/CHINA, http://linkedlifedata.com/resource/pubmed/keyword/Developing Countries, http://linkedlifedata.com/resource/pubmed/keyword/Eastern Asia, http://linkedlifedata.com/resource/pubmed/keyword/Examinations And Diagnoses, http://linkedlifedata.com/resource/pubmed/keyword/Family Planning, http://linkedlifedata.com/resource/pubmed/keyword/Genitalia, http://linkedlifedata.com/resource/pubmed/keyword/Genitalia, Male, http://linkedlifedata.com/resource/pubmed/keyword/Germ Cells, http://linkedlifedata.com/resource/pubmed/keyword/Laboratory Examinations And Diagnoses, http://linkedlifedata.com/resource/pubmed/keyword/Male Sterilization, http://linkedlifedata.com/resource/pubmed/keyword/Physiology, http://linkedlifedata.com/resource/pubmed/keyword/Research Report, http://linkedlifedata.com/resource/pubmed/keyword/Reversibility, http://linkedlifedata.com/resource/pubmed/keyword/SPERMATOZOA, http://linkedlifedata.com/resource/pubmed/keyword/Sterilization, Sexual, http://linkedlifedata.com/resource/pubmed/keyword/Urogenital System, http://linkedlifedata.com/resource/pubmed/keyword/VAS DEFERENS, http://linkedlifedata.com/resource/pubmed/keyword/Vasectomy
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0105-6263
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
181-5
pubmed:dateRevised
2006-11-15
pubmed:otherAbstract
PIP: Vasectomy is a widely used birth control method. Although spermatogenesis continues in the testis after vasectomy, the secretion and absorption functions of the epididymis and proximal vas deferens are changed. Spermatozoa stored in the epididymis may therefore be affected. Some men regret having been sterilized and seek to reverse their vasectomy. While the development of microsurgery has increased the success rate of vasovasostomy, the rate at which such men are able to impregnate fertile women remains only in the 50-58% range. Defects of spermatozoa in the epididymis following vasectomization may contribute to this low fertilizing capacity of men who have attempted to reverse the effects of their vasectomy. This study assessed the condition of spermatozoa from the proximal vas deferens of 67 men after vasectomy. Subjects were aged 25-40 years with duration of vasectomy three months to ten years with no chronic medical illnesses. The men's fluids were collected from both proximal vas deferens by cannulating the vas deferens at the time of vasectomy reversal. Selected sperm parameters were then analyzed after incubation of the spermatozoa for 30 minutes at 37 degrees Celsius. Sperm concentration in the proximal vas from vasectomized men was significantly higher than that of fertile men and was maintained at a constant level independent of the duration of vas obstruction. The means of sperm motility, spermatozoa with morphology, sperm viability, and hypo-osmotic swelling test were statistically lower than the respective values for normally fertile men, while no significant correlation was observed between the duration of vas obstruction and the above semen parameters. In 46.4% of vas fluids all spermatozoa were immotile, especially after three years of vasectomy, suggesting that immotile spermatozoa in the proximal vas fluids at the time of vasectomy reversal may be an important factor for predicting semen quality and fertilizing ability after vasovasostomy. There were not, however, significant differences in the results of sperm-cervical mucus penetration test between spermatozoa from vasectomized and fertile men. It is noted that the presence of antisperm bodies on the spermatozoa from the vas of vasectomized men, as determined by the immunobead test, may partially explain the lower pregnancy rate after vasovasostomy, with spermatozoa parameters potentially closely reflecting those in the cauda epididymis after vasectomy.
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Analysis of spermatozoa from the proximal vas deferens of vasectomized men.
pubmed:affiliation
Family Planning Research Institute of Guangdong, Guangzhou, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't