Linked Life Data

7994907

Source:http://linkedlifedata.com/resource/pubmed/id/7994907

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1995-1-19
pubmed:abstractText
An attempt was made to determine whether addition of purified autoantibodies against U1-ribonucleoprotein (RNP) and negatively charged molecules (cardiolipin and double-stranded (ds) DNA) to cultures of peripheral blood monocytes could enhance the synthesis of cytokines in patients with MCTD and normal healthy volunteers. It was found that: (i) at the baseline, levels of cytokines such as IL-1 alpha, IL-1 beta and IL-6 extracellularly released by or associated with monocytes were significantly higher in MCTD patients than in normal subjects; (ii) addition of antibodies against U1-RNP to cultures of MCTD monocytes resulted in a significant overall increase of the released and cell-associated IL-1 alpha, IL-1 beta and IL-6. On the other hand, addition of antibodies against cardiolipin or dsDNA to cultures of MCTD monocytes resulted in a significant increase of released and/or cell-associated IL-1 alpha and IL-1 beta; (iii) addition of these autoantibodies to cultures of normal monocytes resulted in a significant overall increase of released and cell-associated IL-1 alpha, IL-1 beta and IL-6. The extent of enhancement of cytokines released by or associated with monocytes was greater in normal subjects than in MCTD patients; (iv) a F(ab')2 preparation of autoantibodies against U1-RNP also enhanced the level of released and cell-associated IL-1 alpha. Our findings that both autoantibodies against U1-RNP and negatively charged molecules were able to enhance the synthesis of cytokines by monocytes suggest that these autoantibodies might cause derangement of endothelial cells and lead to proliferative vasculopathy, which is a characteristic of pulmonary hypertension in MCTD.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7994907-1333924, http://linkedlifedata.com/resource/pubmed/commentcorrection/7994907-1451330, http://linkedlifedata.com/resource/pubmed/commentcorrection/7994907-1599524, http://linkedlifedata.com/resource/pubmed/commentcorrection/7994907-1690096, http://linkedlifedata.com/resource/pubmed/commentcorrection/7994907-1901780, http://linkedlifedata.com/resource/pubmed/commentcorrection/7994907-1994864, http://linkedlifedata.com/resource/pubmed/commentcorrection/7994907-2123932, http://linkedlifedata.com/resource/pubmed/commentcorrection/7994907-2332868, http://linkedlifedata.com/resource/pubmed/commentcorrection/7994907-3082573, http://linkedlifedata.com/resource/pubmed/commentcorrection/7994907-3491707, http://linkedlifedata.com/resource/pubmed/commentcorrection/7994907-3872755, http://linkedlifedata.com/resource/pubmed/commentcorrection/7994907-4621694, http://linkedlifedata.com/resource/pubmed/commentcorrection/7994907-6171283, http://linkedlifedata.com/resource/pubmed/commentcorrection/7994907-6179522, http://linkedlifedata.com/resource/pubmed/commentcorrection/7994907-625325, http://linkedlifedata.com/resource/pubmed/commentcorrection/7994907-6966469, http://linkedlifedata.com/resource/pubmed/commentcorrection/7994907-8370166
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0009-9104
pubmed:author
pubmed:issnType
Print
pubmed:volume
98
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
427-33
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Enhanced synthesis of cytokines by peripheral blood monocytes cultured in the presence of autoantibodies against U1-ribonucleoprotein and/or negatively charged molecules: implication in the pathogenesis of pulmonary hypertension in mixed connective tissue disease (MCTD).
pubmed:affiliation
Division of Immunology, International Medical Centre of Japan, Tokyo.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't