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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1995-1-12
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pubmed:abstractText |
HT-29 sublines and Caco-2 clones were analyzed for the expression of cytochrome P-450 3A. The enzyme was found to be expressed in differentiated HT-29 cells selected by resistance to methotrexate and in one of seven Caco-2 clones, TC7. Its expression parallels the differentiation process, with highest levels being observed at late confluency. P-450 3A mRNA and protein patterns, as well as subcellular distribution, are intermediate between those observed in human adult intestine and fetal liver.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
355
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pubmed:geneSymbol |
CYP3A
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
247-50
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7988682-Blotting, Northern,
pubmed-meshheading:7988682-Blotting, Western,
pubmed-meshheading:7988682-Carcinoma,
pubmed-meshheading:7988682-Cell Compartmentation,
pubmed-meshheading:7988682-Cell Differentiation,
pubmed-meshheading:7988682-Clone Cells,
pubmed-meshheading:7988682-Colonic Neoplasms,
pubmed-meshheading:7988682-Cytochrome P-450 CYP2E1,
pubmed-meshheading:7988682-Cytochrome P-450 Enzyme System,
pubmed-meshheading:7988682-Drug Resistance,
pubmed-meshheading:7988682-Fluorescent Antibody Technique,
pubmed-meshheading:7988682-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:7988682-Humans,
pubmed-meshheading:7988682-Methotrexate,
pubmed-meshheading:7988682-Mixed Function Oxygenases,
pubmed-meshheading:7988682-RNA, Messenger,
pubmed-meshheading:7988682-Selection, Genetic,
pubmed-meshheading:7988682-Tumor Cells, Cultured
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pubmed:year |
1994
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pubmed:articleTitle |
Expression of cytochrome P-450 3A in HT29-MTX cells and Caco-2 clone TC7.
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pubmed:affiliation |
Unité de Recherches de Biochimie Pharmacologique et Métabolique (INSERM U75), Centre Hospitalo-Universitaire Necker-Enfants Malades, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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