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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
23
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pubmed:dateCreated |
1995-1-9
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pubmed:abstractText |
Protein splicing involves the excision of an internal domain from a precursor protein and the ligation of the external domains so as to generate two new proteins. Study of this process has recently been facilitated by the isolation of a precursor and a branched intermediate from a thermophilic protein splicing element expressed in a foreign protein context. Two aspects of protein splicing are examined in this paper. We demonstrate a succinimide at the C-terminus of the spliced internal protein, implicating cyclization of asparagine in resolution of the branched intermediate, and we identify an alkali-labile bond in the branched intermediate. A revised protein splicing model based on these experimental results is presented.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7988548-1330319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7988548-1417861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7988548-1423588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7988548-1475179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7988548-1534148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7988548-1608969,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7988548-1909321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7988548-2139027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7988548-2146742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7988548-3198609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7988548-3805008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7988548-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7988548-7508863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7988548-8065257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7988548-8165123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7988548-8269515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7988548-8274142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7988548-8352597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7988548-8463270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7988548-8495192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7988548-8508780
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5517-22
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7988548-Alkalies,
pubmed-meshheading:7988548-Amino Acid Sequence,
pubmed-meshheading:7988548-Base Sequence,
pubmed-meshheading:7988548-Conserved Sequence,
pubmed-meshheading:7988548-DNA Primers,
pubmed-meshheading:7988548-Molecular Sequence Data,
pubmed-meshheading:7988548-Protein Processing, Post-Translational,
pubmed-meshheading:7988548-Proteins,
pubmed-meshheading:7988548-Sequence Homology, Amino Acid,
pubmed-meshheading:7988548-Succinimides
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pubmed:year |
1994
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pubmed:articleTitle |
Protein splicing: an analysis of the branched intermediate and its resolution by succinimide formation.
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pubmed:affiliation |
New England Biolabs, Inc., Beverly, MA 01915.
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pubmed:publicationType |
Journal Article
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