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| pubmed-article:7986032 | lifeskim:mentions | umls-concept:C0581406 | lld:lifeskim |
| pubmed-article:7986032 | lifeskim:mentions | umls-concept:C1522664 | lld:lifeskim |
| pubmed-article:7986032 | lifeskim:mentions | umls-concept:C0452849 | lld:lifeskim |
| pubmed-article:7986032 | lifeskim:mentions | umls-concept:C1550638 | lld:lifeskim |
| pubmed-article:7986032 | lifeskim:mentions | umls-concept:C1552596 | lld:lifeskim |
| pubmed-article:7986032 | lifeskim:mentions | umls-concept:C0439831 | lld:lifeskim |
| pubmed-article:7986032 | lifeskim:mentions | umls-concept:C1707927 | lld:lifeskim |
| pubmed-article:7986032 | lifeskim:mentions | umls-concept:C1947931 | lld:lifeskim |
| pubmed-article:7986032 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:7986032 | pubmed:dateCreated | 1995-1-5 | lld:pubmed |
| pubmed-article:7986032 | pubmed:abstractText | Artificially inoculated Escherichia coli O157:H7 was directly enumerated in ground beef and beef exudate, without enrichment or selection, by the antibody-direct epifluorescent filter technique (Ab-DEFT). The total assay time of the Ab-DEFT was less than 1 h. The beef was homogenized, treated for 15 min with trypsin and Triton X-100, and passed through a 5-microns-pore-size prefilter and then through a 0.2-microns-pore-size black polycarbonate filter. The final filter was stained directly with fluorescein-labeled anti-O157 polyclonal antibody, rinsed, and examined by epifluorescence microscopy. The sensitivity of the Ab-DEFT was compared with that of a standard enrichment culture technique. Both methods reliably determined the presence of the pathogen in beef at 16 CFU/g. The Ab-DEFT was also useful for quantifying the pathogen and monitoring its growth in beef. | lld:pubmed |
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| pubmed-article:7986032 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:7986032 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:7986032 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7986032 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:7986032 | pubmed:issn | 0099-2240 | lld:pubmed |
| pubmed-article:7986032 | pubmed:author | pubmed-author:TortorelloM... | lld:pubmed |
| pubmed-article:7986032 | pubmed:author | pubmed-author:StewartD SDS | lld:pubmed |
| pubmed-article:7986032 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7986032 | pubmed:volume | 60 | lld:pubmed |
| pubmed-article:7986032 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7986032 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7986032 | pubmed:pagination | 3553-9 | lld:pubmed |
| pubmed-article:7986032 | pubmed:dateRevised | 2010-9-13 | lld:pubmed |
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| pubmed-article:7986032 | pubmed:meshHeading | pubmed-meshheading:7986032-... | lld:pubmed |
| pubmed-article:7986032 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7986032 | pubmed:articleTitle | Antibody-direct epifluorescent filter technique for rapid, direct enumeration of Escherichia coli O157:H7 in beef. | lld:pubmed |
| pubmed-article:7986032 | pubmed:affiliation | National Center for Food Safety and Technology, U.S. Food and Drug Administration, Summit-Argo, Illinois 60501. | lld:pubmed |
| pubmed-article:7986032 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7986032 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:7986032 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7986032 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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