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rdf:type |
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lifeskim:mentions |
umls-concept:C0180860,
umls-concept:C0376624,
umls-concept:C0439831,
umls-concept:C0439851,
umls-concept:C0449851,
umls-concept:C0452849,
umls-concept:C0581406,
umls-concept:C1522664,
umls-concept:C1546637,
umls-concept:C1550638,
umls-concept:C1552596,
umls-concept:C1704449,
umls-concept:C1704684,
umls-concept:C1707927,
umls-concept:C1947931
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pubmed:issue |
10
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pubmed:dateCreated |
1995-1-5
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pubmed:abstractText |
Artificially inoculated Escherichia coli O157:H7 was directly enumerated in ground beef and beef exudate, without enrichment or selection, by the antibody-direct epifluorescent filter technique (Ab-DEFT). The total assay time of the Ab-DEFT was less than 1 h. The beef was homogenized, treated for 15 min with trypsin and Triton X-100, and passed through a 5-microns-pore-size prefilter and then through a 0.2-microns-pore-size black polycarbonate filter. The final filter was stained directly with fluorescein-labeled anti-O157 polyclonal antibody, rinsed, and examined by epifluorescence microscopy. The sensitivity of the Ab-DEFT was compared with that of a standard enrichment culture technique. Both methods reliably determined the presence of the pathogen in beef at 16 CFU/g. The Ab-DEFT was also useful for quantifying the pathogen and monitoring its growth in beef.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7986032-1280180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7986032-1369498,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7986032-1559010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7986032-1583138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7986032-16345515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7986032-16345938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7986032-1691146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7986032-1993773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7986032-2187844,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7986032-3060018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7986032-3069862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7986032-3300914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7986032-3519658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7986032-4581469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7986032-6548470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7986032-6655531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7986032-7149713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7986032-8368828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7986032-8501228
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0099-2240
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
60
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3553-9
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:7986032-Animals,
pubmed-meshheading:7986032-Antibodies, Bacterial,
pubmed-meshheading:7986032-Antibody Specificity,
pubmed-meshheading:7986032-Cattle,
pubmed-meshheading:7986032-Colony Count, Microbial,
pubmed-meshheading:7986032-Escherichia coli,
pubmed-meshheading:7986032-Evaluation Studies as Topic,
pubmed-meshheading:7986032-Filtration,
pubmed-meshheading:7986032-Fluorescent Antibody Technique,
pubmed-meshheading:7986032-Meat,
pubmed-meshheading:7986032-Sensitivity and Specificity
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pubmed:year |
1994
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pubmed:articleTitle |
Antibody-direct epifluorescent filter technique for rapid, direct enumeration of Escherichia coli O157:H7 in beef.
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pubmed:affiliation |
National Center for Food Safety and Technology, U.S. Food and Drug Administration, Summit-Argo, Illinois 60501.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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