Linked Life Data

7985762

Source:http://linkedlifedata.com/resource/pubmed/id/7985762

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1995-1-3
pubmed:abstractText
Previous studies have shown that serum from humans with fascioliasis had precipitating antibodies that identified a line of precipitation as specific for Fasciola. This Fasciola-specific band was designated arc 2. The specificity of a Fasciola-specific anti-arc 2 antiserum, evaluated by Western blot analysis, reveals a mosaic of antigens with molecular weights in the range of 10-43 kD. In the current study, the antibodies reacting with a subset of antigens in the 10-21-kD range were eluted from nitrocellulose and used to probe an F. hepatica cDNA library. Four clones were purified and amplified by the polymerase chain reaction. The DNA hybridizations among these clones revealed different groups of clones derived from different mRNAs. The finding of multiple genes supports the observation that F. hepatica arc 2 is a mosaic of antigens. The studies show that molecular cloning technology can be used to identify potential genes, each of which encode distinct antigens that may be used for the specific immunodiagnosis of fascioliasis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0002-9637
pubmed:author
pubmed:issnType
Print
pubmed:volume
51
pubmed:geneSymbol
arc 2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
684-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Identification of genes that encode Fasciola-specific arc 2 antigens.
pubmed:affiliation
Department of Pathology and Laboratory Medicine, University of Puerto Rico School of Medicine, San Juan.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't