rdf:type |
|
lifeskim:mentions |
umls-concept:C0012854,
umls-concept:C0025663,
umls-concept:C0034760,
umls-concept:C0205155,
umls-concept:C0439855,
umls-concept:C0487602,
umls-concept:C1521827,
umls-concept:C1522472,
umls-concept:C1523987,
umls-concept:C1551341,
umls-concept:C1552858,
umls-concept:C1552923,
umls-concept:C1552924,
umls-concept:C1705191
|
pubmed:issue |
12
|
pubmed:dateCreated |
1995-1-5
|
pubmed:abstractText |
Batches of osmium-ammine (OA) complex vary considerably in staining properties when used in a Feulgen-like reaction for selective staining of DNA-containing structures. An alternative procedure for preparation of Schiff-like reagent from OA complex is described. It is based on generation of H2SO3, respectively SO2, within the OA solution and ensures more favorable conditions for production of a Schiff-like stain than bubbling with SO2 does. The method is reproducible and yields high staining intensity. Data obtained suggest that the ability of OA complex to produce Feulgen-like staining is strongly influenced by variations in its chemical composition. Their unfavorable effect can be overcome by selecting suitable conditions for preparation of a Schiff-like reagent. Conditions for obtaining specific and sensitive Feulgen-like staining are determined.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Dec
|
pubmed:issn |
0022-1554
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
42
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1643-9
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:7983365-Acrylic Resins,
pubmed-meshheading:7983365-Animals,
pubmed-meshheading:7983365-DNA,
pubmed-meshheading:7983365-Indicators and Reagents,
pubmed-meshheading:7983365-Liver,
pubmed-meshheading:7983365-Male,
pubmed-meshheading:7983365-Osmium Compounds,
pubmed-meshheading:7983365-Quaternary Ammonium Compounds,
pubmed-meshheading:7983365-Rats,
pubmed-meshheading:7983365-Rats, Wistar,
pubmed-meshheading:7983365-Sulfhydryl Compounds
|
pubmed:year |
1994
|
pubmed:articleTitle |
An alternative method for preparation of Schiff-like reagent from osmium-ammine complex for selective staining of DNA on thin Lowicryl sections.
|
pubmed:affiliation |
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|