Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
49
|
| pubmed:dateCreated |
1995-1-4
|
| pubmed:abstractText |
The Gsl-5 gene mapped on mouse chromosome 19 controls the expression of IV6 beta Gal beta 1-4(Fuc alpha 1-3)GlcNAc, IV3 beta Gal-Gb4Cer in mouse kidney through regulation of the activity of UDP-GlcNAc:IV3 beta Gal-Gb4Cer beta-1,6-Glc-NAc transferase (GNT), which transfers GlcNAc to the C-6 position of GalNAc of IV3 beta Gal-Gb4Cer (GL-X). Here we report that GNT has been purified to apparent homogeneity from mouse kidney by means of preparation of a microsomal fraction, solubilization with Triton X-100, and sequential column chromatographies on CM-Sepharose, UDP-hexanolamine-Sepharose, and Gg4Ose-Aminocellulo-fine. GNT purified 11,000-fold from the microsomal fraction exhibited a specific activity of 26.15 mumol/min/mg protein and an apparent molecular mass of 50 kDa on SDS-polyacrylamide gel electrophoresis. The Km values for UDP-GlcNAc and GL-X were 0.36 and 0.11 mM, respectively. Among glycolipids tested as substrates, GL-X was the best and Gg4Cer the next best, but Lc3Cer, Gb4Cer, and GM1 did not act as a substrate. GNT was also able to transfer GlcNAc to Gal beta 1-3GalNAc beta 1-benzyl and Gal beta 1-3GalNAc alpha 1-p-nitrophenyl at C-6 of GalNAc through beta linkage but not to GlcNAc beta-3GalNAc alpha 1-p-nitrophenyl. The purified GNT was digested with lysyl endopeptidase, and four peptides generated were sequenced. The sequence of four peptides spanning 35 amino acid residues in total exhibited 80% homology with that of the reported human core 2 GlcNAc transferase.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
9
|
| pubmed:volume |
269
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
31143-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7983056-Amino Acid Sequence,
pubmed-meshheading:7983056-Animals,
pubmed-meshheading:7983056-Carbohydrate Sequence,
pubmed-meshheading:7983056-Chromatography, Gel,
pubmed-meshheading:7983056-Chromatography, Ion Exchange,
pubmed-meshheading:7983056-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:7983056-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:7983056-Humans,
pubmed-meshheading:7983056-Hydrogen-Ion Concentration,
pubmed-meshheading:7983056-Kidney,
pubmed-meshheading:7983056-Mice,
pubmed-meshheading:7983056-Microsomes,
pubmed-meshheading:7983056-Molecular Sequence Data,
pubmed-meshheading:7983056-N-Acetylglucosaminyltransferases,
pubmed-meshheading:7983056-Polymorphism, Genetic,
pubmed-meshheading:7983056-Sequence Homology, Amino Acid,
pubmed-meshheading:7983056-Substrate Specificity
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Purification and characterization of UDP-GlcNAc:IV3 beta Gal-Gb4Cer beta-1,6-GlcNAc transferase from mouse kidney.
|
| pubmed:affiliation |
Department of Membrane Biochemistry, Tokyo Metropolitan Institute of Medical Science, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|