| pubmed-article:7982915 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7982915 | lifeskim:mentions | umls-concept:C0063695 | lld:lifeskim |
| pubmed-article:7982915 | lifeskim:mentions | umls-concept:C0129533 | lld:lifeskim |
| pubmed-article:7982915 | lifeskim:mentions | umls-concept:C1327287 | lld:lifeskim |
| pubmed-article:7982915 | lifeskim:mentions | umls-concept:C0013879 | lld:lifeskim |
| pubmed-article:7982915 | lifeskim:mentions | umls-concept:C1314939 | lld:lifeskim |
| pubmed-article:7982915 | lifeskim:mentions | umls-concept:C0070750 | lld:lifeskim |
| pubmed-article:7982915 | pubmed:issue | 48 | lld:pubmed |
| pubmed-article:7982915 | pubmed:dateCreated | 1994-12-30 | lld:pubmed |
| pubmed-article:7982915 | pubmed:abstractText | Treatment of cells with interferon (IFN)-gamma or phorbol myristate acetate (PMA) induces up-regulation of the level of intercellular adhesion molecule-1 (ICAM-1; CD54) mRNA by stabilization of an otherwise labile mRNA. Here, we have generated various deletion mutants of ICAM-1 and stably transfected them into the murine fibroblast Ltk- cells that express no endogenous ICAM-1 or -2 (CD102) in an effort to define the regions within ICAM-1 mRNA responsive to IFN-gamma or PMA. Induction of ICAM-1 mRNA in the transfected L cells by the treatment with IFN-gamma revealed that the truncation of the region of ICAM-1 mRNA encoding the cytoplasmic domain made it non-responsive to IFN-gamma whereas all other regions were dispensable. In contrast, PMA-induced accumulation of ICAM-1 mRNA required the 3'-untranslated region (UTR). To further elucidate the role of these regions in mRNA destabilization and responsiveness to IFN-gamma and PMA, ICAM-2 mRNA that is stable and not responsive to IFN-gamma or PMA was used as a reporter gene. The putative IFN-gamma-responsive region of ICAM-1 mRNA encoding its cytoplasmic domain rendered it unstable and responsive to IFN-gamma but not PMA. Conversely, the 3'-UTR of ICAM-1 fused with ICAM-2 mRNA also made it unstable and responsive to PMA but not IFN-gamma. Half-life analysis showed that the induction of these chimeric mRNAs by IFN-gamma and PMA was due, at least in part, to the prolongation of their turnover rate. These results taken together demonstrate that two distinct regions of ICAM-1 mRNA regulate its stability, one encoding the cytoplasmic domain and responsive to IFN-gamma and the other in the 3'-UTR and responsive to PMA. | lld:pubmed |
| pubmed-article:7982915 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7982915 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7982915 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7982915 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7982915 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7982915 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7982915 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7982915 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7982915 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7982915 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7982915 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:7982915 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:7982915 | pubmed:author | pubmed-author:TakeiFF | lld:pubmed |
| pubmed-article:7982915 | pubmed:author | pubmed-author:OhiRR | lld:pubmed |
| pubmed-article:7982915 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7982915 | pubmed:day | 2 | lld:pubmed |
| pubmed-article:7982915 | pubmed:volume | 269 | lld:pubmed |
| pubmed-article:7982915 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7982915 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7982915 | pubmed:pagination | 30117-20 | lld:pubmed |
| pubmed-article:7982915 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:7982915 | pubmed:meshHeading | pubmed-meshheading:7982915-... | lld:pubmed |
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| pubmed-article:7982915 | pubmed:meshHeading | pubmed-meshheading:7982915-... | lld:pubmed |
| pubmed-article:7982915 | pubmed:meshHeading | pubmed-meshheading:7982915-... | lld:pubmed |
| pubmed-article:7982915 | pubmed:meshHeading | pubmed-meshheading:7982915-... | lld:pubmed |
| pubmed-article:7982915 | pubmed:meshHeading | pubmed-meshheading:7982915-... | lld:pubmed |
| pubmed-article:7982915 | pubmed:meshHeading | pubmed-meshheading:7982915-... | lld:pubmed |
| pubmed-article:7982915 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7982915 | pubmed:articleTitle | Interferon-gamma- and phorbol myristate acetate-responsive elements involved in intercellular adhesion molecule-1 mRNA stabilization. | lld:pubmed |
| pubmed-article:7982915 | pubmed:affiliation | Department of Medical Genetics, University of British Columbia, Vancouver, Canada. | lld:pubmed |
| pubmed-article:7982915 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7982915 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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