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rdf:type |
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lifeskim:mentions |
umls-concept:C0081757,
umls-concept:C0205224,
umls-concept:C0449432,
umls-concept:C1167622,
umls-concept:C1179435,
umls-concept:C1513112,
umls-concept:C1524073,
umls-concept:C1548799,
umls-concept:C1622418,
umls-concept:C1705248,
umls-concept:C1744214
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pubmed:issue |
48
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|
pubmed:dateCreated |
1994-12-30
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pubmed:databankReference |
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pubmed:abstractText |
Nef is a 27-kDa myristylated protein conserved in most human immunodeficiency virus (HIV)-1, HIV-2, and simian immunodeficiency virus isolates. Simian immunodeficiency virus Nef is required in macaques for both high viral load and full pathological effects. Nef down-regulates the cell surface expression of CD4 by a post-translational mechanism that is not yet fully elucidated. We have used the yeast two-hybrid system to identify cellular proteins that interact with Nef. A cDNA was isolated which encodes a COOH-terminal fragment of human beta-COP, a major coat component of non-clathrin-coated vesicles. Nef and beta-COP interacted in vitro and were found to be physically associated in HIV-1-infected cells by co-immunoprecipitation. These observations suggest that beta-COP might be one of the cellular mediators of Nef function in HIV-1-infected cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9258
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
2
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pubmed:volume |
269
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
30073-6
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:7982906-Amino Acid Sequence,
pubmed-meshheading:7982906-Animals,
pubmed-meshheading:7982906-Base Sequence,
pubmed-meshheading:7982906-Cloning, Molecular,
pubmed-meshheading:7982906-Coated Pits, Cell-Membrane,
pubmed-meshheading:7982906-Coatomer Protein,
pubmed-meshheading:7982906-Conserved Sequence,
pubmed-meshheading:7982906-DNA, Complementary,
pubmed-meshheading:7982906-DNA Primers,
pubmed-meshheading:7982906-Gene Products, nef,
pubmed-meshheading:7982906-HIV-1,
pubmed-meshheading:7982906-HIV-2,
pubmed-meshheading:7982906-Humans,
pubmed-meshheading:7982906-Macaca,
pubmed-meshheading:7982906-Membrane Proteins,
pubmed-meshheading:7982906-Microtubule-Associated Proteins,
pubmed-meshheading:7982906-Molecular Sequence Data,
pubmed-meshheading:7982906-Polymerase Chain Reaction,
pubmed-meshheading:7982906-Rats,
pubmed-meshheading:7982906-Sequence Homology, Amino Acid,
pubmed-meshheading:7982906-Simian immunodeficiency virus,
pubmed-meshheading:7982906-nef Gene Products, Human Immunodeficiency Virus
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pubmed:year |
1994
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|
pubmed:articleTitle |
Physical interaction of the HIV-1 Nef protein with beta-COP, a component of non-clathrin-coated vesicles essential for membrane traffic.
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pubmed:affiliation |
Unité 332, INSERM, Paris, France.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|
