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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1995-1-4
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pubmed:abstractText |
Activation of protein kinase C (PKC) has been shown to regulate electrolyte transport in rabbit small intestine. We investigated the types of PKC isoforms in rabbit ileal villus and crypt cells and the potential for phorbol 12-myristate 13-acetate (PMA) to induce translocation of the PKC from the cytosol to the plasma membrane. Our results indicate that there are at least three PKC isoforms, alpha, epsilon and zeta, are present in both villus and crypt enterocytes. Acute treatment with PMA resulted in translocation of the PKC-alpha from the cytosol to the membrane fraction in both cell types. Prolonged exposure of the villus cells to PMA resulted in a significant progressive decrement in PKC-alpha, suggesting down regulation. Since PMA treatment results in translocation, this isoform may be involved in the regulation of electrolyte transport in the rabbit ileum.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:author |
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pubmed:volume |
108
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
171-8
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:7981979-Amino Acid Sequence,
pubmed-meshheading:7981979-Animals,
pubmed-meshheading:7981979-Biological Transport,
pubmed-meshheading:7981979-Blotting, Western,
pubmed-meshheading:7981979-Cell Fractionation,
pubmed-meshheading:7981979-Cell Membrane,
pubmed-meshheading:7981979-Cytosol,
pubmed-meshheading:7981979-Down-Regulation,
pubmed-meshheading:7981979-Electrolytes,
pubmed-meshheading:7981979-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:7981979-Enzyme Activation,
pubmed-meshheading:7981979-Enzyme Induction,
pubmed-meshheading:7981979-Ileum,
pubmed-meshheading:7981979-Immune Sera,
pubmed-meshheading:7981979-Isoenzymes,
pubmed-meshheading:7981979-Male,
pubmed-meshheading:7981979-Microvilli,
pubmed-meshheading:7981979-Molecular Sequence Data,
pubmed-meshheading:7981979-Protein Kinase C,
pubmed-meshheading:7981979-Rabbits,
pubmed-meshheading:7981979-Tetradecanoylphorbol Acetate
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pubmed:year |
1994
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pubmed:articleTitle |
Identification of protein kinase C-alpha, epsilon and zeta in rabbit ileal enterocytes.
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pubmed:affiliation |
Department of Medicine, Winthrop-University Hospital, State University of New York at Stony Brook, Mineola 11501.
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pubmed:publicationType |
Journal Article
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