Linked Life Data

7978649

Source:http://linkedlifedata.com/resource/pubmed/id/7978649

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1994-12-28
pubmed:abstractText
Lymphocyte functions in cattle affected with leukocyte adhesion deficiency (LAD, termed BLAD in cattle) were evaluated by lymphocyte markers, blastogenic response, and immunoglobulin concentrations; mononuclear phagocyte functions were assessed by chemotactic and luminol-dependent chemiluminescent (CL) responses to determine the effects of impaired expression of leukocyte CD18 on mononuclear cell functions. Deficient CD18 expression on lymphocytes and mononuclear phagocytes from cattle with BLAD was clearly detected by use of flow cytometric analysis. There were no significant differences in the population of peanut agglutinin (PNA)-positive and surface immunoglobulin-bearing blood lymphocytes from clinically normal cattle and cattle with BLAD, as determined by flow cytometric analysis. Lymphocytes from cattle with BLAD had strong mitogen-induced blastogenic responses, which were greater than those from controls. Adherence of mononuclear phagocytes from cattle with BLAD was markedly impaired, and their chemotactic responses had diminished values, compared with those of controls. Luminol-dependent CL of mononuclear phagocytes from affected cattle, stimulated by opsonized zymosan, had significantly (P < 0.01) decreased values, compared with those of controls. Concentrations of IgG were markedly increased in serum from cattle with BLAD, compared with those in controls. These results indicated that impaired expression of leukocyte CD18 has marked effects on adhering activity of mononuclear phagocytes, and significantly inhibits CL response of mononuclear phagocytes mediated by inactivated-complement 3b-dependent functions. High selective immunoglobulin concentrations indicated that lymphocytes of B-cell lineage may have normal function.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0002-9645
pubmed:author
pubmed:issnType
Print
pubmed:volume
55
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1101-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Analysis of mononuclear cell functions in Holstein cattle with leukocyte adhesion deficiency.
pubmed:affiliation
Department of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't