Linked Life Data

7978315

Source:http://linkedlifedata.com/resource/pubmed/id/7978315

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
1994-12-22
pubmed:abstractText
Evidence for the accumulation and storage of ionized duplex DNA molecules in a quadrupole ion trap is presented. Aqueous solutions of complementary single-strand molecules of DNA were annealed to form duplexes in solution and subjected to electrospray ionization. The ions liberated in this process were transported through an atmosphere/vacuum interface and injected into a quadrupole ion trap operated with a bath gas present at a pressure of 1 mTorr. Despite the roughly 2 order of magnitude poorer signal levels noted for electrospray of aqueous solutions relative to those observed for single-strand oligonucleotides in methanol solutions, aqueous solutions were used to avoid denaturing the duplexes. Ion trap mass spectra are reported here for duplexes consisting of two complementary 20-mer single strands and two complementary 10-mers. Tandem mass spectrometry results are also reported for the 10-mer duplex. These results are significant in that they indicate that the ions are kinetically stable under the ion injection, storage, and mass analysis conditions of the quadrupole ion trap operated with a relatively high pressure of bath gas. The tools of ion trap mass spectrometry can therefore be applied to this important class of compounds.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0003-2700
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3416-22
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Accumulation and storage of ionized duplex DNA molecules in a quadrupole ion trap.
pubmed:affiliation
Biology Division, Oak Ridge National Laboratory, Tennessee 37831-6365.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't