Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1994-11-29
|
| pubmed:abstractText |
Normal human epidermis is a rich source of biologically active interleukin-1 alpha (IL-1 alpha). Keratinocytes both synthesize this cytokine and respond to it via cell surface receptors (IL-1R), suggesting that the IL-1 system may play an important role in normal epidermal physiology and inflammation. In this study, we have examined the expression of IL-1R in normal and psoriatic epidermis, as judged at a functional level by the capacity to bind 125I-labeled IL-1 alpha (the principal IL-1 species present in epidermis) and by immunostaining with antibodies specific for each species of IL-1R. IL-1R was not readily detectable by either technique in normal, freshly isolated human epidermis. However, in lesional psoriasis or normal epidermis after 24 hours of organ culture, expression of IL-1R was dramatically induced, especially in basal keratinocytes. Immunostaining and antibody blocking studies demonstrated the induced IL-1R to be the type II species, a nonsignal transducing molecule previously demonstrated only on leukocytes. The Ka of this receptor was comparable to that previously demonstrated in vitro. mRNA for both species of IL-1R could be demonstrated by reverse transcriptase-polymerase chain reaction in fresh and cultured epidermis. These in vivo findings were confirmed in culture, where normal human keratinocytes expressed few IL-1R at rest but large numbers of type II IL-1R after activation by phorbol ester or interferon-gamma. We conclude that under resting conditions, epidermal expression of IL-1R is low. However, the potential for keratinocytes in vivo to express large numbers of the nonsignal transducing type II IL-1R is evident from both organ cultured and psoriatic epidermis. The in vitro induction of keratinocyte IL-1R by interferon-gamma suggests that this cytokine may be involved in the induction of type II IL-1R in inflammatory skin disease. The presence of bioactive IL-1 in epidermis, coupled with the inducible expression of the decoy type II IL-1R, indicates the existence of a highly regulated system of autocrine stimulation of keratinocytes by IL-1.
|
| pubmed:grant | |
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7977638-1372029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7977638-1530822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7977638-1690903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7977638-1826616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7977638-1833184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7977638-2141048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7977638-2254445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7977638-2355179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7977638-2460504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7977638-2465548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7977638-2470087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7977638-2478449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7977638-2969618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7977638-2997285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7977638-3007615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7977638-3018088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7977638-8332913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7977638-8428929
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0002-9440
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
145
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1048-56
|
| pubmed:dateRevised |
2009-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:7977638-Autoradiography,
pubmed-meshheading:7977638-Base Sequence,
pubmed-meshheading:7977638-DNA Primers,
pubmed-meshheading:7977638-Epidermis,
pubmed-meshheading:7977638-Humans,
pubmed-meshheading:7977638-Immunoenzyme Techniques,
pubmed-meshheading:7977638-Interleukin-1,
pubmed-meshheading:7977638-Keratinocytes,
pubmed-meshheading:7977638-Molecular Sequence Data,
pubmed-meshheading:7977638-Organ Culture Techniques,
pubmed-meshheading:7977638-Polymerase Chain Reaction,
pubmed-meshheading:7977638-Psoriasis,
pubmed-meshheading:7977638-RNA, Messenger,
pubmed-meshheading:7977638-Receptors, Interleukin-1
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Detection of interleukin-1 receptors in human epidermis. Induction of the type II receptor after organ culture and in psoriasis.
|
| pubmed:affiliation |
Harvard Skin Disease Research Center, Harvard Medical School, Brigham and Women's Hospital, Boston, Massachusetts.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|