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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1994-12-12
|
| pubmed:databankReference | |
| pubmed:abstractText |
The 10A1 murine leukemia virus induces tumors that lack lineage-specific markers found on myeloid, T-cell, and B-cell lineages. Either erythroid or multipotent stem cells can have this phenotype; therefore we have used fluorescence-activated cell sorter analysis with either multipotent stem cell markers or markers found on lineage-restricted precursors to differentiate between these two possibilities. The results showed that tumors induced by 10A1 expressed multipotent stem cell markers as well as some lineage-restricted precursor markers. To further study the tumor phenotype, we analyzed total RNAs from 10A1-induced tumors by Northern blotting for c-kit, erythropoietin receptor, and T-cell gamma receptor mRNAs. Most of the tumors contained these mRNAs, which are characteristic of early hematopoietic cells. These results are consistent with the hypothesis that 10A1-induced tumor cells are early multipotent hematopoietic stem cells. Southern blot analysis revealed that 14 of 14 10A1-induced tumor cell DNAs examined contained MuLV integrations into the fli-1 gene. The results strongly suggested that promoter insertion into fli-1 is required for tumor formation.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fli1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Protein c-fli-1,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0042-6822
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
205
|
| pubmed:geneSymbol |
fli-1
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
563-8
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7975258-Animals,
pubmed-meshheading:7975258-Antigens, Differentiation,
pubmed-meshheading:7975258-Base Sequence,
pubmed-meshheading:7975258-Cell Separation,
pubmed-meshheading:7975258-DNA-Binding Proteins,
pubmed-meshheading:7975258-Flow Cytometry,
pubmed-meshheading:7975258-Hematopoietic Stem Cells,
pubmed-meshheading:7975258-Leukemia, Experimental,
pubmed-meshheading:7975258-Leukemia Virus, Murine,
pubmed-meshheading:7975258-Mice,
pubmed-meshheading:7975258-Molecular Sequence Data,
pubmed-meshheading:7975258-Proto-Oncogene Protein c-fli-1,
pubmed-meshheading:7975258-Proto-Oncogene Proteins,
pubmed-meshheading:7975258-Proto-Oncogenes,
pubmed-meshheading:7975258-Retroviridae Infections,
pubmed-meshheading:7975258-Trans-Activators,
pubmed-meshheading:7975258-Tumor Virus Infections,
pubmed-meshheading:7975258-Virus Integration
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
10A1 MuLV induces a murine leukemia that expresses hematopoietic stem cell markers by a mechanism that includes fli-1 integration.
|
| pubmed:affiliation |
Laboratory of Molecular Virology and Carcinogenesis, PRI/DynCorp, Inc., NCI-Frederick Cancer Research and Development Center, Maryland 21702-1201.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|