Linked Life Data

7972494

Source:http://linkedlifedata.com/resource/pubmed/id/7972494

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1994-11-30
pubmed:abstractText
Expression and physiological effects of the root-inducing rolB gene of Agrobacterium rhizogenes T-DNA were studied simultaneously in tobacco (Nicotiana tabacum) mesophyll protoplasts. The kinetic study of the expression of rolB mRNA following exogenous auxin application showed that auxin transiently stimulated rolB expression, with mRNA levels starting to accumulate 6 to 9 h after auxin was supplied and increasing 300-fold after 12 to 18 h. The parallel study of the auxin sensitivity of rolB-transformed protoplasts, as assayed by their electrical response to the hormone, showed that the auxin treatment generated an increase in sensitivity by a factor of up to 100,000, whereas in untransformed protoplasts the same auxin treatment induced an increase in auxin sensitivity that never exceeded 30- to 50-fold. This reflects a strong cooperative effect of auxin and rolB in transformed protoplasts. Surprisingly, the maximal increase in sensitivity was observed several hours before the maximal accumulation of rolB mRNA, suggesting that the dramatic control of auxin sensitivity by auxin in rolB-transformed protoplasts requires only low levels of rolB expression. Antibodies directed against ZmER-abp1, the major auxin-binding protein from maize, differentially altered the auxin sensitivity of the electrical response of rolB-transformed and normal protoplasts. This suggests that alterations of the auxin reception-transduction pathway at the plasma membrane of rolB-transformed protoplasts may account for their increased auxin sensitivity.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
105
pubmed:geneSymbol
rolB
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1209-15
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:7972494-Agrobacterium tumefaciens, pubmed-meshheading:7972494-Bacterial Proteins, pubmed-meshheading:7972494-Base Sequence, pubmed-meshheading:7972494-Blotting, Southern, pubmed-meshheading:7972494-DNA, Bacterial, pubmed-meshheading:7972494-DNA, Plant, pubmed-meshheading:7972494-DNA Primers, pubmed-meshheading:7972494-Gene Expression, pubmed-meshheading:7972494-Genes, Bacterial, pubmed-meshheading:7972494-Indoleacetic Acids, pubmed-meshheading:7972494-Molecular Sequence Data, pubmed-meshheading:7972494-Plant Leaves, pubmed-meshheading:7972494-Plants, Toxic, pubmed-meshheading:7972494-Polymerase Chain Reaction, pubmed-meshheading:7972494-Protoplasts, pubmed-meshheading:7972494-RNA, Messenger, pubmed-meshheading:7972494-Recombinant Fusion Proteins, pubmed-meshheading:7972494-Rhizobium, pubmed-meshheading:7972494-Sensitivity and Specificity, pubmed-meshheading:7972494-Tobacco
pubmed:year
1994
pubmed:articleTitle
Alterations of auxin perception in rolB-transformed tobacco protoplasts. Time course of rolB mRNA expression and increase in auxin sensitivity reveal multiple control by auxin.
pubmed:affiliation
Institut des Sciences Végétales, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't