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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
1994-12-19
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pubmed:abstractText |
The DNase I hypersensitive site 5' HS2 of the human beta-globin locus control region confers position-independent, high-level expression on the human beta-globin gene in transgenic mice. When a 5' HS2 beta-globin construct is flanked by retroviral vector sequences derived from Moloney Murine Leukemia Virus (MoMLV), expression of the beta-globin gene is severely inhibited. Apparently, one or more elements within the MoMLV genome is capable of repressing transcription of the human beta-globin gene in transgenic mice. A construct lacking the retroviral enhancer also fails to express the beta-globin gene, indicating that this region of the virus is not essential for repression. Further analysis may permit the identification of specific viral sequences that inhibit gene expression; these sequences could then be deleted or mutated to produce improved viral vectors.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7971278-1570310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7971278-1847517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7971278-1902783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7971278-1987488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7971278-2000371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7971278-2357965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7971278-2631796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7971278-2721958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7971278-2780563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7971278-2797152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7971278-2992937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7971278-3458176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7971278-3690667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7971278-8152905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7971278-8348617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7971278-8428573
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4477-81
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7971278-Animals,
pubmed-meshheading:7971278-Blotting, Southern,
pubmed-meshheading:7971278-Deoxyribonuclease I,
pubmed-meshheading:7971278-Enhancer Elements, Genetic,
pubmed-meshheading:7971278-Gene Expression Regulation,
pubmed-meshheading:7971278-Gene Transfer Techniques,
pubmed-meshheading:7971278-Genetic Vectors,
pubmed-meshheading:7971278-Globins,
pubmed-meshheading:7971278-Humans,
pubmed-meshheading:7971278-Mice,
pubmed-meshheading:7971278-Mice, Transgenic,
pubmed-meshheading:7971278-Microinjections,
pubmed-meshheading:7971278-Moloney murine leukemia virus,
pubmed-meshheading:7971278-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:7971278-Transcription, Genetic
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pubmed:year |
1994
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pubmed:articleTitle |
Retroviral vector sequences inhibit human beta-globin gene expression in transgenic mice.
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pubmed:affiliation |
Department of Biochemistry and Molecular Genetics, School of Medicine, University of Alabama at Birmingham 35294.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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