Linked Life Data

7968514

Source:http://linkedlifedata.com/resource/pubmed/id/7968514

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-11-29
pubmed:databankReference
pubmed:abstractText
During phosphate-limited growth the marine phycoerythrin-containing picoplanktonic cyanobacterium Synechococcus sp. WH7803 synthesizes novel polypeptides, including two abundant species of 100 kDa and 32 kDa. The 32 kDa polypeptide was localized to the cell wall, although in a related strain, Synechococcus sp. WH8103, it could be detected in both the cell wall fraction and the periplasm. The gene (designated pstS) encoding this polypeptide was cloned and shown to be present in a single copy. The deduced amino acid sequence indicated a polypeptide consisting of 326 amino acids with a calculated M(r) of 33,763. Comparison of this sequence with that obtained by microsequencing the N-terminus of the 32 kDa polypeptide showed that the mature protein was synthesized as a precursor, the first 24 amino acid residues being cleaved between two alanine residues at positions 24 and 25. The amino acid sequence of the mature polypeptide showed 35% identity and 52% similarity to the periplasmic phosphate-binding protein (PstS) from Escherichia coli, including three regions of much stronger homology which, by comparison with E. coli PstS, are directly involved in phosphate binding. Northern blot analysis revealed a pstS transcript of 1.2 kb in RNA extracted from cells grown in Pi-replete conditions and one of 1.4 kb in considerably increased abundance under Pi-depleted conditions. Homologues of the pstS gene were detected in other marine phycoerythrin-containing Synechococcus strains, but not in freshwater or halotolerant species.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0950-382X
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:geneSymbol
pstS
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
181-91
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7968514-Amino Acid Sequence, pubmed-meshheading:7968514-Base Sequence, pubmed-meshheading:7968514-Biological Transport, pubmed-meshheading:7968514-Carrier Proteins, pubmed-meshheading:7968514-Cell Wall, pubmed-meshheading:7968514-Cyanobacteria, pubmed-meshheading:7968514-Escherichia coli, pubmed-meshheading:7968514-Escherichia coli Proteins, pubmed-meshheading:7968514-Gene Expression Regulation, Bacterial, pubmed-meshheading:7968514-Membrane Proteins, pubmed-meshheading:7968514-Molecular Sequence Data, pubmed-meshheading:7968514-Periplasmic Binding Proteins, pubmed-meshheading:7968514-Phosphate-Binding Proteins, pubmed-meshheading:7968514-Phosphates, pubmed-meshheading:7968514-Phycoerythrin, pubmed-meshheading:7968514-Sequence Alignment, pubmed-meshheading:7968514-Sequence Homology, Amino Acid, pubmed-meshheading:7968514-Species Specificity
pubmed:year
1993
pubmed:articleTitle
The response of the picoplanktonic marine cyanobacterium Synechococcus species WH7803 to phosphate starvation involves a protein homologous to the periplasmic phosphate-binding protein of Escherichia coli.
pubmed:affiliation
Department of Biological Sciences, University of Warwick, Coventry, UK.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't