Linked Life Data

796723

Source:http://linkedlifedata.com/resource/pubmed/id/796723

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
1977-3-31
pubmed:abstractText
Luria-Delbrück fluctuation tests for the determination of the spontaneous mutation rate to ouabain resistance incultured L5178Y mouse lymphoma cells gave values in the range 0.44 to 1.03 X 10(-7) mutants per cell per generation. Addition of very low, non-toxic, levels of EMS (0.1 mM) and MMS (0.012 mM) 30-48 h before plating in selective medium gave a highly significant increase in the number of ouabain-resistant mutants. Methods for the calculation of spontaneous and induced mutation rates are discussed and a method for the computation of induced mutation rates is described. It is suggested that the modified fluctuation test is a simpler and considerably more sensitive assay for mutagens than the conventional experimental design. Some of its limitations are discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0027-5107
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
377-86
pubmed:dateRevised
2000-12-18
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
The fluctuation test as a more sensitive system for determining induced mutation in L5178Y mouse lymphoma cells.
pubmed:publicationType
Journal Article