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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1994-12-28
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pubmed:abstractText |
1. Investigations were made into the effects of BRL 38227, a potassium channel activator, on ATP-sensitive potassium channels (K+ATP channels) in single fibres dissociated from the flexor digitorum brevis muscle of C57BL/6J mice. 2. In cell-attached patches BRL 38227 (100 microM) caused activation of a glibenclamide-sensitive potassium current. Linear slope conductance of the inward current, partial rectification of the outward current and glibenclamide sensitivity indicate that K+ATP channels are the site of action of BRL 38227. 3. In the absence of ATP at the cytoplasmic side of excised inside-out patches, BRL 38227 caused direct and magnesium-dependent activation of K+ATP channels. The degree of activation diminished with successive applications of BRL 38227. 4. BRL 38227 also caused activation of K+ATP channels in the presence of low (< 100 microM) but not high (1.0 mM) ATP, particularly in patches containing large numbers of channels. 5. BRL 38227 and 5 microM MgATP failed to activate channels following complete run-down. 6. Results show that BRL 38227 caused direct activation of K+ATP in skeletal muscle and that this was mediated through a magnesium-dependent binding site rather than alleviation of inhibition by competitive displacement of ATP from the inhibitory site.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-1488275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-1501145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-1630885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-1643077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-1649991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-1706203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-1717778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-1750525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-1786512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-1786796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-1834361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-1890633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-1910095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-1917907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-1941637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-1996617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-2086765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-2151053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-2184534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-2230831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-2280794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-2282934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-2301614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-2411918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-2428009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-2436138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-2442362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-2443648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-2456760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-2456785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-2513734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-2601686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-2676059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-2725710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-2810349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-2813039,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-6270629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7965862-8459449
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
478 Pt 3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
523-32
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7965862-Adenosine Triphosphate,
pubmed-meshheading:7965862-Animals,
pubmed-meshheading:7965862-Benzopyrans,
pubmed-meshheading:7965862-Cromakalim,
pubmed-meshheading:7965862-Female,
pubmed-meshheading:7965862-Glyburide,
pubmed-meshheading:7965862-Male,
pubmed-meshheading:7965862-Mice,
pubmed-meshheading:7965862-Mice, Inbred C57BL,
pubmed-meshheading:7965862-Muscle, Skeletal,
pubmed-meshheading:7965862-Muscle Fibers, Skeletal,
pubmed-meshheading:7965862-Patch-Clamp Techniques,
pubmed-meshheading:7965862-Potassium Channels,
pubmed-meshheading:7965862-Pyrroles
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pubmed:year |
1994
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pubmed:articleTitle |
Mechanism of action of a K+ channel activator BRL 38227 on ATP-sensitive K+ channels in mouse skeletal muscle fibres.
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pubmed:affiliation |
Division of Neuroscience, School of Biological Sciences, University of Manchester.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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