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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1994-12-23
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L26451,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L26452,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L26453,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L26454,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L26455,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L26456,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L26457,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L26458,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L29035,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L29036,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L29037,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L29038
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pubmed:abstractText |
To examine T cell receptor (TCR) diversity involved in the memory response to a persistent human pathogen, we determined nucleotide sequences encoding TCR-alpha and -beta chains from HLA-B8-restricted, CD8+ cytotoxic T cell clones specific for an immunodominant epitope (FLRGRAYGL) in Epstein-Barr virus (EBV) nuclear antigen 3. Herein, we show that identical TCR protein sequences are used by clones from each of four healthy unrelated virus carriers; a clone from a fifth varied conservatively at only two residues. This dominant selection of alpha and beta chain rearrangements suggest that a persistent viral infection can select for a highly focused memory response and indicates a strong bias in gene segment usage and recombination. A novel double-step semiquantitative polymerase chain reaction (PCR) procedure and direct sequencing of amplified TCR cDNA from fresh lymphocytes derived from three HLA-B8 individuals detected transcripts specific for the conserved beta chain in an EBV-seropositive donor but not in two seronegative donors. This report describes an unprecedented degree of conservation in TCR selected in response to a natural persistent infection.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-1309938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-1311263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-1370414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-1371525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-1377222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-1378424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-1409706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-1515141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-1689749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-1752360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-1923806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-1940807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-2026880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-2455603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-2993909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-3043226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-3866244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-6204924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-6319303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-7511682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-7903854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-8145043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-8391985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-8397778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7964506-8477809
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-1007
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pubmed:author |
pubmed-author:ArgaetV PVP,
pubmed-author:BurrowsS RSR,
pubmed-author:DoolanD LDL,
pubmed-author:KieffEE,
pubmed-author:KurillaM GMG,
pubmed-author:MiskoI SIS,
pubmed-author:MossD JDJ,
pubmed-author:SchmidtC WCW,
pubmed-author:SculleyT BTB,
pubmed-author:SilinsS LSL,
pubmed-author:SuhrbierAA
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
180
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2335-40
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7964506-Amino Acid Sequence,
pubmed-meshheading:7964506-Base Sequence,
pubmed-meshheading:7964506-Carrier State,
pubmed-meshheading:7964506-Clone Cells,
pubmed-meshheading:7964506-Conserved Sequence,
pubmed-meshheading:7964506-DNA Primers,
pubmed-meshheading:7964506-Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor,
pubmed-meshheading:7964506-Gene Rearrangement, beta-Chain T-Cell Antigen Receptor,
pubmed-meshheading:7964506-Herpesviridae Infections,
pubmed-meshheading:7964506-Herpesvirus 4, Human,
pubmed-meshheading:7964506-Humans,
pubmed-meshheading:7964506-Molecular Sequence Data,
pubmed-meshheading:7964506-Polymerase Chain Reaction,
pubmed-meshheading:7964506-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:7964506-T-Lymphocytes,
pubmed-meshheading:7964506-Tumor Virus Infections
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pubmed:year |
1994
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pubmed:articleTitle |
Dominant selection of an invariant T cell antigen receptor in response to persistent infection by Epstein-Barr virus.
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pubmed:affiliation |
Queensland Institute of Medical Research, Bancroft Centre, Brisbane, Australia.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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