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| pubmed-article:7961491 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
| pubmed-article:7961491 | lifeskim:mentions | umls-concept:C0004793 | lld:lifeskim |
| pubmed-article:7961491 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:7961491 | lifeskim:mentions | umls-concept:C0079866 | lld:lifeskim |
| pubmed-article:7961491 | lifeskim:mentions | umls-concept:C0805701 | lld:lifeskim |
| pubmed-article:7961491 | lifeskim:mentions | umls-concept:C1283195 | lld:lifeskim |
| pubmed-article:7961491 | lifeskim:mentions | umls-concept:C0207108 | lld:lifeskim |
| pubmed-article:7961491 | pubmed:issue | 23 | lld:pubmed |
| pubmed-article:7961491 | pubmed:dateCreated | 1994-12-27 | lld:pubmed |
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| pubmed-article:7961491 | pubmed:abstractText | The gene encoding penicillin-binding protein 4 (PBP 4) of Bacillus subtilis, pbpD, was cloned by two independent methods. PBP 4 was purified, and the amino acid sequence of a cyanogen bromide digestion product was used to design an oligonucleotide probe for identification of the gene. An oligonucleotide probe designed to hybridize to genes encoding class A high-molecular-weight PBPs also identified this gene. DNA sequence analysis of the cloned DNA revealed that (i) the amino acid sequence of PBP 4 was similar to those of other class A high-molecular-weight PBPs and (ii) pbpD appeared to be cotranscribed with a downstream gene (termed orf2) of unknown function. The orf2 gene is followed by an apparent non-protein-coding region which exhibits nucleotide sequence similarity with at least two other regions of the chromosome and which has a high potential for secondary structure formation. Mutations in pbpD resulted in the disappearance of PBP 4 but had no obvious effect on growth, cell division, sporulation, spore heat resistance, or spore germination. Expression of a transcriptional fusion of pbpD to lacZ increased throughout growth, decreased during sporulation, and was induced approximately 45 min into spore germination. A single transcription start site was detected just upstream of pbpD. The pbpD locus was mapped to the 275 to 280 degrees region of the chromosomal genetic map. | lld:pubmed |
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| pubmed-article:7961491 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7961491 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7961491 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7961491 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7961491 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7961491 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7961491 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7961491 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:7961491 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7961491 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:7961491 | pubmed:issn | 0021-9193 | lld:pubmed |
| pubmed-article:7961491 | pubmed:author | pubmed-author:SetlowPP | lld:pubmed |
| pubmed-article:7961491 | pubmed:author | pubmed-author:PophamD LDL | lld:pubmed |
| pubmed-article:7961491 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7961491 | pubmed:volume | 176 | lld:pubmed |
| pubmed-article:7961491 | pubmed:geneSymbol | pbpD | lld:pubmed |
| pubmed-article:7961491 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7961491 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7961491 | pubmed:pagination | 7197-205 | lld:pubmed |
| pubmed-article:7961491 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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