Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
1994-12-27
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L10630, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L13867, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L17438, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L25426, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L28837, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M62809, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M90527, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M90528, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U11882, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U11883, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X02163, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X02164, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X67870, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X70356, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X73124, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Z22737
pubmed:abstractText
The gene encoding penicillin-binding protein 4 (PBP 4) of Bacillus subtilis, pbpD, was cloned by two independent methods. PBP 4 was purified, and the amino acid sequence of a cyanogen bromide digestion product was used to design an oligonucleotide probe for identification of the gene. An oligonucleotide probe designed to hybridize to genes encoding class A high-molecular-weight PBPs also identified this gene. DNA sequence analysis of the cloned DNA revealed that (i) the amino acid sequence of PBP 4 was similar to those of other class A high-molecular-weight PBPs and (ii) pbpD appeared to be cotranscribed with a downstream gene (termed orf2) of unknown function. The orf2 gene is followed by an apparent non-protein-coding region which exhibits nucleotide sequence similarity with at least two other regions of the chromosome and which has a high potential for secondary structure formation. Mutations in pbpD resulted in the disappearance of PBP 4 but had no obvious effect on growth, cell division, sporulation, spore heat resistance, or spore germination. Expression of a transcriptional fusion of pbpD to lacZ increased throughout growth, decreased during sporulation, and was induced approximately 45 min into spore germination. A single transcription start site was detected just upstream of pbpD. The pbpD locus was mapped to the 275 to 280 degrees region of the chromosomal genetic map.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-1537791, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-1548223, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-1624445, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-1629150, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-1644769, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-1713281, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-1741619, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-1809831, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-1902213, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-1908013, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-1936941, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-2104615, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-2231712, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-2251112, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-2599354, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-2826251, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-2832368, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-3015878, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-3087956, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-3285892, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-330236, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-3330753, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-345275, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-3906031, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-3917999, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-4344991, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-4995746, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-6304019, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-6378385, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-6378622, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-6402492, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-6810758, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-7110312, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-7934828, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-8244929, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-8289242, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-8335642, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-8355609, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-8407792, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-8449926, http://linkedlifedata.com/resource/pubmed/commentcorrection/7961491-8491712
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
176
pubmed:geneSymbol
pbpD
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7197-205
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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