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| pubmed-article:7961454 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:7961454 | lifeskim:mentions | umls-concept:C0037083 | lld:lifeskim |
| pubmed-article:7961454 | lifeskim:mentions | umls-concept:C1514562 | lld:lifeskim |
| pubmed-article:7961454 | lifeskim:mentions | umls-concept:C1883221 | lld:lifeskim |
| pubmed-article:7961454 | lifeskim:mentions | umls-concept:C1710082 | lld:lifeskim |
| pubmed-article:7961454 | lifeskim:mentions | umls-concept:C1372999 | lld:lifeskim |
| pubmed-article:7961454 | lifeskim:mentions | umls-concept:C0949707 | lld:lifeskim |
| pubmed-article:7961454 | lifeskim:mentions | umls-concept:C0040661 | lld:lifeskim |
| pubmed-article:7961454 | lifeskim:mentions | umls-concept:C1705162 | lld:lifeskim |
| pubmed-article:7961454 | lifeskim:mentions | umls-concept:C1883204 | lld:lifeskim |
| pubmed-article:7961454 | lifeskim:mentions | umls-concept:C1880389 | lld:lifeskim |
| pubmed-article:7961454 | pubmed:issue | 22 | lld:pubmed |
| pubmed-article:7961454 | pubmed:dateCreated | 1994-12-16 | lld:pubmed |
| pubmed-article:7961454 | pubmed:abstractText | The phototaxis-deficient mutant of Halobacterium salinarium, Pho81, lacks both sensory rhodopsin I (SR-I) and its putative transducer protein HtrI, according to immunoblotting and spectroscopic criteria. From restriction analysis and selected DNA sequencing, we have determined that the SR-I- HtrI- phenotype results from an insertion of a 520-bp transposable element, ISH2, into the coding region of the SR-I apoprotein gene sopI and deletion of 11 kbp upstream of ISH2 including the first 164 bp of sopI and the entire htrI gene. SR-I and HtrI expression as well as full phototaxis sensitivity are restored by transformation with a halobacterial plasmid carrying the htrI-sopI gene pair and their upstream promoter region. An internal deletion of a portion of htrI encoding the putative methylation and signaling domains of HtrI (253 residues) prevents the restoration of phototaxis, providing further evidence for the role of HtrI as a transducer for SR-I. Analysis of flash-induced photochemical reactions of SR-I over a range of pH shows that the partially deleted HtrI maintains SR-I interactions sites responsible for modulation of the SR-I photocycle. | lld:pubmed |
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| pubmed-article:7961454 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:7961454 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:7961454 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7961454 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:7961454 | pubmed:issn | 0021-9193 | lld:pubmed |
| pubmed-article:7961454 | pubmed:author | pubmed-author:PaiM RMR | lld:pubmed |
| pubmed-article:7961454 | pubmed:author | pubmed-author:SpudichE NEN | lld:pubmed |
| pubmed-article:7961454 | pubmed:author | pubmed-author:SpudichJ LJL | lld:pubmed |
| pubmed-article:7961454 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7961454 | pubmed:volume | 176 | lld:pubmed |
| pubmed-article:7961454 | pubmed:geneSymbol | sopI | lld:pubmed |
| pubmed-article:7961454 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7961454 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7961454 | pubmed:pagination | 6931-5 | lld:pubmed |
| pubmed-article:7961454 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:7961454 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7961454 | pubmed:articleTitle | Identification of distinct domains for signaling and receptor interaction of the sensory rhodopsin I transducer, HtrI. | lld:pubmed |
| pubmed-article:7961454 | pubmed:affiliation | Department of Microbiology and Molecular Genetics, University of Texas Medical School Health Science Center, Houston 77030. | lld:pubmed |
| pubmed-article:7961454 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7961454 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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