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rdf:type |
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lifeskim:mentions |
umls-concept:C0020792,
umls-concept:C0037083,
umls-concept:C0040661,
umls-concept:C0949707,
umls-concept:C1372999,
umls-concept:C1514562,
umls-concept:C1705162,
umls-concept:C1710082,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221
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pubmed:issue |
22
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pubmed:dateCreated |
1994-12-16
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pubmed:abstractText |
The phototaxis-deficient mutant of Halobacterium salinarium, Pho81, lacks both sensory rhodopsin I (SR-I) and its putative transducer protein HtrI, according to immunoblotting and spectroscopic criteria. From restriction analysis and selected DNA sequencing, we have determined that the SR-I- HtrI- phenotype results from an insertion of a 520-bp transposable element, ISH2, into the coding region of the SR-I apoprotein gene sopI and deletion of 11 kbp upstream of ISH2 including the first 164 bp of sopI and the entire htrI gene. SR-I and HtrI expression as well as full phototaxis sensitivity are restored by transformation with a halobacterial plasmid carrying the htrI-sopI gene pair and their upstream promoter region. An internal deletion of a portion of htrI encoding the putative methylation and signaling domains of HtrI (253 residues) prevents the restoration of phototaxis, providing further evidence for the role of HtrI as a transducer for SR-I. Analysis of flash-induced photochemical reactions of SR-I over a range of pH shows that the partially deleted HtrI maintains SR-I interactions sites responsible for modulation of the SR-I photocycle.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7961454-12049093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7961454-1465418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7961454-1956318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7961454-1992477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7961454-2448875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7961454-2682623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7961454-2748598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7961454-3410829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7961454-3449857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7961454-3790692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7961454-3818549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7961454-4044522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7961454-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7961454-6300900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7961454-6504161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7961454-7350544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7961454-8187768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7961454-8312493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7961454-8344242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7961454-8344892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7961454-8446619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7961454-8475097
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Archaeal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Bacteriorhodopsins,
http://linkedlifedata.com/resource/pubmed/chemical/Halorhodopsins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SRI protein, Halobacterium,
http://linkedlifedata.com/resource/pubmed/chemical/Sensory Rhodopsins,
http://linkedlifedata.com/resource/pubmed/chemical/htrI protein, Halobacterium...
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
176
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pubmed:geneSymbol |
sopI
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6931-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7961454-Antibodies, Bacterial,
pubmed-meshheading:7961454-Archaeal Proteins,
pubmed-meshheading:7961454-Bacterial Proteins,
pubmed-meshheading:7961454-Bacteriorhodopsins,
pubmed-meshheading:7961454-Base Sequence,
pubmed-meshheading:7961454-Cell Movement,
pubmed-meshheading:7961454-DNA Mutational Analysis,
pubmed-meshheading:7961454-Escherichia coli,
pubmed-meshheading:7961454-Halobacterium,
pubmed-meshheading:7961454-Halorhodopsins,
pubmed-meshheading:7961454-Light,
pubmed-meshheading:7961454-Membrane Proteins,
pubmed-meshheading:7961454-Molecular Sequence Data,
pubmed-meshheading:7961454-Recombinant Proteins,
pubmed-meshheading:7961454-Sensory Rhodopsins,
pubmed-meshheading:7961454-Signal Transduction,
pubmed-meshheading:7961454-Structure-Activity Relationship,
pubmed-meshheading:7961454-Transformation, Genetic
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pubmed:year |
1994
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pubmed:articleTitle |
Identification of distinct domains for signaling and receptor interaction of the sensory rhodopsin I transducer, HtrI.
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pubmed:affiliation |
Department of Microbiology and Molecular Genetics, University of Texas Medical School Health Science Center, Houston 77030.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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