Linked Life Data

7957179

Source:http://linkedlifedata.com/resource/pubmed/id/7957179

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-11-28
pubmed:abstractText
In yeast, as in higher eukaryotic cells, sphingolipids are essential membrane components. The yeast, Saccharomyces cerevisiae, contains three classes of sphingolipids, inositolphosphorylceramide (InsPCer), mannosylinositolphosphorylceramide (ManInsPCer) and mannosyldiinositolphosphorylceramide (ManPIns2PCer). As a prerequisite to localize these sphingolipids in subcellular membranes, authentic standards of the respective lipids were isolaed and characterized using biochemical methods and electrospray ionization mass spectrometry. The complete set of yeast subcellular membranes was isolated at high purity, and sphingolipids were extracted. InsPCer, ManInsPCer, and ManPIns2PCer were separated by thin-layer chromatography, stained and densitometrically scanned along with the respective standards. These methods enable a complete overview of the subcellular distribution of yeast sphingolipids to be obtained, as far as is known, for the first time. InsPCer was highly enriched in Golgi and vacuolar membranes, whereas the largest amounts of ManInsPCer and ManPIns2PCer were found in the plasma membrane. The presence of inositol-containing sphingolipids in organelles of the protein-secretory pathway strongly supports the notion that protein secretion and intracellular trafficking of sphingolipids are linked processes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
225
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
641-9
pubmed:dateRevised
2007-7-23
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Characterization, quantification and subcellular localization of inositol-containing sphingolipids of the yeast, Saccharomyces cerevisiae.
pubmed:affiliation
Institut für Biochemie und Lebensmittelchemie, Technische Universität Graz, Austria.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't