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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1994-12-16
|
| pubmed:abstractText |
We have used in situ hybridization and Northern blot analysis with oligonucleotide probe to characterize the site of pituitary adenylate cyclase-activating polypeptide (PACAP) synthesis in the rat testis. We observed strong hybridization signal in one third of the cross-sections of the seminiferous tubules, whereas some tubules were devoid of hybridization signal, thus suggesting that PACAP mRNA is expressed in a stage-specific manner. More detailed analysis showed that PACAP mRNA was present in round spermatids at stages III-VII of the cycle. Northern blot hybridization to RNAs extracted from samples of seminiferous tubules at different stages of the epithelial cycle confirmed that expression of PACAP mRNA is restricted to specific stages of the cycle. The highest amount of PACAP mRNA was detected at stages V to early -VII of the cycle, whereas very low levels of mRNA were present at stages I-II and IX-XIV. The present results demonstrate that PACAP mRNA is expressed in the developing germ cells. This suggests that PACAP may function as a paracrine or autocrine regulatory factor for the Sertoli and germ cells, with a specific function during early spermiogenesis, shortly before the onset of nuclear elongation, at the last period of haploid gene activity.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0013-7227
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
135
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2291-4
|
| pubmed:dateRevised |
2005-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:7956953-Animals,
pubmed-meshheading:7956953-Blotting, Northern,
pubmed-meshheading:7956953-Gene Expression Regulation,
pubmed-meshheading:7956953-In Situ Hybridization,
pubmed-meshheading:7956953-Male,
pubmed-meshheading:7956953-Neuropeptides,
pubmed-meshheading:7956953-Pituitary Adenylate Cyclase-Activating Polypeptide,
pubmed-meshheading:7956953-RNA, Messenger,
pubmed-meshheading:7956953-Rats,
pubmed-meshheading:7956953-Rats, Sprague-Dawley,
pubmed-meshheading:7956953-Seminiferous Tubules,
pubmed-meshheading:7956953-Spermatogenesis,
pubmed-meshheading:7956953-Testis
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Stage-specific expression of pituitary adenylate cyclase-activating polypeptide (PACAP) mRNA in the rat seminiferous tubules.
|
| pubmed:affiliation |
Department of Biomedical Sciences, University of Tampere, Finland.
|
| pubmed:publicationType |
Journal Article
|