Linked Life Data

7955369

Source:http://linkedlifedata.com/resource/pubmed/id/7955369

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11 Pt 1
pubmed:dateCreated
1994-12-8
pubmed:abstractText
We have developed a flow-injection system with colorimetric detection to measure 1,5-anhydro-D-glucitol in serum. Serum samples are directly and serially injected into a clean-up column every 3.5 min to remove interferences before the enzymatic reaction. 1,5-Anhydro-D-glucitol, after being passed through the column, is oxidized by immobilized pyranose oxidase (EC 1.1.3.10), and the hydrogen peroxide produced reacts with the chromogen substrate in the presence of immobilized horseradish peroxidase (EC 1.11.1.7) to form Bindshedler's Green. The detection limit was 1.2 mumol/L (1.2 pmol). The correlation between results obtained with the present system (y) and gas chromatography-mass spectrometry (GC-MS) (x) in samples containing < 30 mumol/L 1,5-anhydro-D-glucitol, including many samples from patients with diabetes mellitus, was y = 0.975x-0.111 mumol/L (r = 0.997), which was superior to that obtained between the enzymatic and GC-MS methods. Our system needs only to be set up; it runs without any manual pretreatment, assays 17 samples/h, and shows imprecision (CV) of < 2%.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0009-9147
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2006-12
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Fully automated flow-injection system for quantifying 1,5-anhydro-D-glucitol in serum.
pubmed:affiliation
Takasaki Research Laboratories, Nippon Kayaku Co., Ltd., Gunma, Japan.
pubmed:publicationType
Journal Article, Comparative Study