Linked Life Data

7952054

Source:http://linkedlifedata.com/resource/pubmed/id/7952054

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-12-6
pubmed:abstractText
Turnover of plasma free fatty acids (FFAs) can be determined from the palmitate enrichment of plasma after administration of analogues labeled with stable isotopes. We studied the conditions to measure both the concentration and the 13C enrichment of plasma palmitate by gas chromatography-mass spectrometry (GC-MS) using crude extracts. The method used plasma extraction after addition of heptadecanoic acid as internal standard and methylation with diazomethane. Subsequently the samples were analyzed by GC-MS. Plasma palmitate levels determined with this simplified method did not differ statistically from those obtained by a more "classical" procedure using FFA separation from other plasma lipids. Palmitic acid turnover rates (Ra) were evaluated in the steady-state period, in two normal subjects after 90 min infusion with [1-13C]palmitate bound to human albumin. The rate of appearance (Ra) was found to be 0.92 and 1.08 mmol kg-1 min-1, which is in good agreement with the turnover rate previously reported for normal subjects. Sample preparation and GC-MS analysis by the proposed procedure are simple and rapid and thus the method appears to be particularly useful in clinical studies where numerous samples have to be analyzed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1572-6495
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
657
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-7
pubmed:dateRevised
2007-10-16
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Fast gas chromatographic-mass spectrometric method for the evaluation of plasma fatty acid turnover using [1-13C]palmitate.
pubmed:affiliation
Scientific Institute H.S. Raffaele, Milan, Italy.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't