Linked Life Data

7952029

Source:http://linkedlifedata.com/resource/pubmed/id/7952029

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-11-30
pubmed:abstractText
A procedure for the assay of nitrile hydratase and amidase activity by high-performance liquid chromatography is described. The method can be used to assay the intermediate compounds resulting from the hydrolysis of adiponitrile into adipic acid, and to determine the kinetics of the hydrolysis of these compounds using whole cells and enzyme extracts. The precision of the method makes it suitable for the determination of the enzyme parameters: Km and Vm (nitrile hydratase and amidase). Using cyanovaleramide as substrate, Km and Vm were respectively 370 mM and 2060 U/mg for nitrile hydratase and 6.6 mM and 33 U/mg for amidase.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1572-6495
pubmed:author
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
656
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
197-202
pubmed:dateRevised
2007-10-16
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Application of high-performance liquid chromatography to the study of the biological transformation of adiponitrile.
pubmed:affiliation
Chaire de Microbiologie Industrielle et de Génétique des Microorganismes, Ecole Nationale Supériure Agronomique de Montpellier, France.
pubmed:publicationType
Journal Article