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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1994-12-2
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pubmed:abstractText |
The relationship between heterologous gene copy number, mRNA and secreted protein yields has been studied in Aspergillus niger transformants containing either the hen egg-white lysozyme (HEWL) cDNA gene or a glucoamylase-HEWL gene fusion (incorporating the A. niger glaA gene). The results support a direct relationship between HEWL gene copy number, mRNA and secreted HEWL protein levels at low (< 25) copy numbers. High protein yields are associated with multiple copies of the recombinant gene at a single site. Fusion of the HEWL gene to the glucoamylase gene resulted in higher steady-state levels of heterologous mRNA. Transformants with the HEWL cDNA alone exhibited a ten-fold higher mRNA:protein ratio than transformants with the gene fusion indicating that post-transcriptional events significantly affect final secreted protein yields.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0962-8819
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:geneSymbol |
glaA
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
297-303
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:7951332-Animals,
pubmed-meshheading:7951332-Aspergillus niger,
pubmed-meshheading:7951332-Base Sequence,
pubmed-meshheading:7951332-Blotting, Southern,
pubmed-meshheading:7951332-Chickens,
pubmed-meshheading:7951332-Cloning, Molecular,
pubmed-meshheading:7951332-DNA, Complementary,
pubmed-meshheading:7951332-DNA Primers,
pubmed-meshheading:7951332-Female,
pubmed-meshheading:7951332-Glucan 1,4-alpha-Glucosidase,
pubmed-meshheading:7951332-Molecular Sequence Data,
pubmed-meshheading:7951332-Muramidase,
pubmed-meshheading:7951332-Polymerase Chain Reaction,
pubmed-meshheading:7951332-Protein Biosynthesis,
pubmed-meshheading:7951332-RNA, Messenger,
pubmed-meshheading:7951332-Recombinant Fusion Proteins,
pubmed-meshheading:7951332-Recombinant Proteins,
pubmed-meshheading:7951332-Transcription, Genetic
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pubmed:year |
1994
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pubmed:articleTitle |
Transcriptional and post-transcriptional events affect the production of secreted hen egg white lysozyme by Aspergillus niger.
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pubmed:affiliation |
Department of Genetics and Microbiology, Institute of Food Research, Norwich Research Park, Colney, UK.
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pubmed:publicationType |
Journal Article
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