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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1994-11-28
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pubmed:databankReference |
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pubmed:abstractText |
Antibodies present in a serum obtained from an infertile woman interacted with a 17.5 kD glycoprotein (BS-17 component) extracted from human sperm by Western blot. Polyclonal antibodies were raised against the BS-17 component and used to identify positive staining clones from a human testis lambda gt11 expression library. The cDNA encoding the BS-17 component was isolated and its nucleotide sequence determined. The BS-17 cDNA contained 758 nucleotides with an open reading frame of 558 nucleotides encoding a polypeptide consisting of 186 amino acids. The BS-17 cDNA showed 99.7% homology in 758 nucleotides overlap with the 3' terminus of the gene coding calpastatin and 99.5% identity in 186 amino acid overlap with the carboxyl terminus of calpastatin. The BS-17 component of human sperm corresponds to the carboxyl terminus of calpastatin. This conclusion is supported by the finding that the polyclonal antibodies also interacted with a 84 kD protein corresponding to the M(r) of calpastatin.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1039-9712
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
33
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
245-51
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7951045-Amino Acid Sequence,
pubmed-meshheading:7951045-Antibodies, Monoclonal,
pubmed-meshheading:7951045-Base Sequence,
pubmed-meshheading:7951045-Blotting, Western,
pubmed-meshheading:7951045-Calcium-Binding Proteins,
pubmed-meshheading:7951045-Cloning, Molecular,
pubmed-meshheading:7951045-Cysteine Proteinase Inhibitors,
pubmed-meshheading:7951045-DNA, Complementary,
pubmed-meshheading:7951045-Female,
pubmed-meshheading:7951045-Humans,
pubmed-meshheading:7951045-Immune Sera,
pubmed-meshheading:7951045-Male,
pubmed-meshheading:7951045-Molecular Sequence Data,
pubmed-meshheading:7951045-Molecular Weight,
pubmed-meshheading:7951045-Restriction Mapping,
pubmed-meshheading:7951045-Sequence Homology, Amino Acid,
pubmed-meshheading:7951045-Sequence Homology, Nucleic Acid,
pubmed-meshheading:7951045-Spermatozoa,
pubmed-meshheading:7951045-Testis
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pubmed:year |
1994
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pubmed:articleTitle |
Calpastatin gene in human testis.
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pubmed:affiliation |
National Laboratory of Medical Molecular Biology, Beijing, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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