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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1994-12-12
|
| pubmed:abstractText |
The biosynthesis of neolacto glycosphingolipids is thought to proceed via reactions catalysed by the two enzymes beta 1-3-N-acetylglucosaminyltransferase (beta 1,3GlcNAcT) and beta 1-4 galactosyltransferase (beta 1,4GalT). In general, only the products of the latter enzyme have been isolated from tissues and structurally characterized. Among the GlcNAc beta 1-3-R glycosphingolipids, only lactotrioasylceramide (Lc3Cer, the initial product in the biosynthesis of neolacto glycosphingolipids) has been isolated and structurally characterized. Longer-chain glycosphingolipids with a terminal GlcNAc-beta 1-3-R structure are considered to be intermediates in the synthesis of complex neolacto glycosphingolipids. We have detected a series of GlcNAc beta 1-3-R glycosphingolipids in extracts obtained from human leukocytes isolated from patients with leukaemia using a monoclonal antibody (TE5) which specifically recognizes these compounds. The structures of three of these compounds purified from chronic myelocytic leukaemia (CML) cells have been determined using a combination of enzymatic, immunostaining and chemical methods. The compounds were found to have the following structures: GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1Cer (Lc3Cer) GlcNAc beta 1-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1Cer (nLc5Cer) GlcNAc beta 1-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1Cer (nLc7Cer) A longer-chain compound, apparently nLc9Cer, was also detected. TLC immunostaining analysis of glycosphingolipids isolated from cells obtained from patients with various leukaemias demonstrated that GlcNAc beta 1-3-R glycosphingolipids have a distribution that depends on the stage of differentiation and lineage of the cell population.(ABSTRACT TRUNCATED AT 250 WORDS)
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0959-6658
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
4
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
251-7
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:7949652-Carbohydrate Sequence,
pubmed-meshheading:7949652-Cell Differentiation,
pubmed-meshheading:7949652-Galactosyltransferases,
pubmed-meshheading:7949652-Glycosphingolipids,
pubmed-meshheading:7949652-Humans,
pubmed-meshheading:7949652-Leukemia,
pubmed-meshheading:7949652-Leukemia, Lymphocytic, Chronic, B-Cell,
pubmed-meshheading:7949652-Leukemia, Myelogenous, Chronic, BCR-ABL Positive,
pubmed-meshheading:7949652-Leukemia, Myeloid, Acute,
pubmed-meshheading:7949652-Leukemia, Myelomonocytic, Acute,
pubmed-meshheading:7949652-Methylation,
pubmed-meshheading:7949652-Molecular Sequence Data,
pubmed-meshheading:7949652-N-Acetylglucosaminyltransferases,
pubmed-meshheading:7949652-Precursor Cell Lymphoblastic Leukemia-Lymphoma,
pubmed-meshheading:7949652-Tumor Cells, Cultured
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| pubmed:year |
1994
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| pubmed:articleTitle |
Structural characterization of intermediates in the biosynthetic pathway of neolacto glycosphingolipids: differential expression in human leukaemia cells.
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| pubmed:affiliation |
Department of Chemistry and Biochemistry, San Francisco State University, CA 94132.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|