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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1994-11-23
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pubmed:databankReference |
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pubmed:abstractText |
A human creatine transporter (hCRT-BS2M) cDNA clone was isolated from a human brainstem/spinal cord using a PCR and phage plaque hybridization based technique. This clone included an open reading frame of 1,905 base pairs(bp) within a 2,283bp cDNA. Northern blot hybridization detected the expression of corresponding mRNAs most prominently in the skeletal muscle, heart and kidney. Peptide sequence analysis of the hCRT-BS2M protein product revealed 12 putative transmembrane domains. The predicted protein sequence further demonstrates that the hCRT-BS2M has highly conserved amino acid identity with the other members of the sodium dependent plasma membrane transporter family. Transient expression of the hCRT-BS2M in COS-7 cells demonstrates sodium dependent [14C]creatine uptake with a KM value of 14.9 +/- 3.0 microM (n = 5) that is attenuated by creatine and selective structural analogues of creatine.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
204
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
419-27
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7945388-Amino Acid Sequence,
pubmed-meshheading:7945388-Animals,
pubmed-meshheading:7945388-Base Sequence,
pubmed-meshheading:7945388-Blotting, Northern,
pubmed-meshheading:7945388-Brain Stem,
pubmed-meshheading:7945388-Carrier Proteins,
pubmed-meshheading:7945388-Cell Line,
pubmed-meshheading:7945388-Cercopithecus aethiops,
pubmed-meshheading:7945388-Cloning, Molecular,
pubmed-meshheading:7945388-Creatine,
pubmed-meshheading:7945388-DNA, Complementary,
pubmed-meshheading:7945388-DNA Primers,
pubmed-meshheading:7945388-Gene Expression,
pubmed-meshheading:7945388-Humans,
pubmed-meshheading:7945388-Kidney,
pubmed-meshheading:7945388-Kinetics,
pubmed-meshheading:7945388-Membrane Transport Proteins,
pubmed-meshheading:7945388-Molecular Sequence Data,
pubmed-meshheading:7945388-Oligonucleotides, Antisense,
pubmed-meshheading:7945388-Polymerase Chain Reaction,
pubmed-meshheading:7945388-Rabbits,
pubmed-meshheading:7945388-Rats,
pubmed-meshheading:7945388-Recombinant Proteins,
pubmed-meshheading:7945388-Restriction Mapping,
pubmed-meshheading:7945388-Sequence Homology, Amino Acid,
pubmed-meshheading:7945388-Spinal Cord,
pubmed-meshheading:7945388-Transfection
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pubmed:year |
1994
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pubmed:articleTitle |
The cloning and expression of a human creatine transporter.
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pubmed:affiliation |
Department of Pharmacology, College of Medicine, University of Arizona, Tucson 85724.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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