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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1994-10-31
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pubmed:abstractText |
Rex-1 is a zinc finger-containing gene that is expressed in embryonal carcinoma (EC) cells and embryonic stem (ES) cells. Upon differentiation with retinoic acid (RA), transcription of the Rex-1 gene decreases rapidly. Analysis of the 5'-flanking region of the Rex-1 gene identified a consensus motif for the octamer family of transcription factors that stimulates expression from the Rex-1 promoter. In this report, we utilized gel mobility shift analysis to examine the binding of transcription factors to the Rex-1 octamer motif. F9 EC cells, D3 ES cells, and human NT2/D1 EC cells each from at least two prominent DNA/protein complexes with the octamer motif. Supershift analysis identifies Oct-1 and Oct-3 in these complexes. When F9 EC cells are induced to differentiate by treatment with RA for 48 h, there is a complete loss of the DNA/protein complex containing Oct-3. In contrast, the other DNA/protein complexes, including the DNA/protein complex containing Oct-1, can still be detected. These results provide support for a role of Oct-3 in the transcription of the Rex-1 gene.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hcfc1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Host Cell Factor C1,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Octamer Transcription Factor-1,
http://linkedlifedata.com/resource/pubmed/chemical/Octamer Transcription Factor-3,
http://linkedlifedata.com/resource/pubmed/chemical/Pou2f1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Pou5f1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tretinoin
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
203
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pubmed:geneSymbol |
Rex-1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1795-802
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:7945330-Animals,
pubmed-meshheading:7945330-Base Sequence,
pubmed-meshheading:7945330-Carcinoma, Embryonal,
pubmed-meshheading:7945330-Cell Differentiation,
pubmed-meshheading:7945330-Cell Line,
pubmed-meshheading:7945330-Cell Nucleus,
pubmed-meshheading:7945330-DNA-Binding Proteins,
pubmed-meshheading:7945330-Embryo, Mammalian,
pubmed-meshheading:7945330-Gene Expression,
pubmed-meshheading:7945330-Genes, pX,
pubmed-meshheading:7945330-Host Cell Factor C1,
pubmed-meshheading:7945330-Mice,
pubmed-meshheading:7945330-Molecular Sequence Data,
pubmed-meshheading:7945330-Nuclear Proteins,
pubmed-meshheading:7945330-Octamer Transcription Factor-1,
pubmed-meshheading:7945330-Octamer Transcription Factor-3,
pubmed-meshheading:7945330-Promoter Regions, Genetic,
pubmed-meshheading:7945330-Stem Cells,
pubmed-meshheading:7945330-Transcription, Genetic,
pubmed-meshheading:7945330-Transcription Factors,
pubmed-meshheading:7945330-Tretinoin,
pubmed-meshheading:7945330-Tumor Cells, Cultured
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pubmed:year |
1994
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pubmed:articleTitle |
The octamer motif present in the Rex-1 promoter binds Oct-1 and Oct-3 expressed by EC cells and ES cells.
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pubmed:affiliation |
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha 68198-6805.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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