Linked Life Data

7942273

Source:http://linkedlifedata.com/resource/pubmed/id/7942273

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1994-11-15
pubmed:abstractText
Cytochrome P4501A1 and its associated aryl hydrocarbon hydroxylase activity are highly inducible in the mouse hepatoma cell line, Hepa-1, by substrates of the enzyme and related compounds, such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Mutants of this cell line, deficient in P4501A1 inducibility, were isolated. Some of the mutants show a dominant phenotype. Such mutants may have resulted from a genetic alteration leading to the inappropriate activation of a repressor gene that normally functions to restrict high level inducibility to the liver and certain other organs or to certain developmental stages. One dominant mutant was shown to express a protein that prevents binding of the liganded aryl hydrocarbon (Ah) receptor (which mediates induction of P4501A1) to its recognition sequence in DNA (the xenobiotic responsive element, or XRE). The majority of mutants are recessive, and were assigned to four different complementation groups (which probably correspond to four different genes). Gene A corresponds to the structural gene (Cyp1a-1) for P4501A1. Mutations in genes B, C and D all affect functioning of the Ah receptor. A cDNA for gene C was cloned. The encoded protein (ARNT) is required for ligand-dependent translocation of the Ah receptor to the nucleus and its binding to the XRE. ARNT and the Ah receptor form a heterodimeric complex which binds the XRE in a fashion such that both subunits bind the XRE directly. Both ARNT and the Ah receptor contain basic helix-loop-helix motifs. Such motifs have been identified in several transcription factors that bind DNA as heterodimers or homodimers. The roles of the proteins corresponding to the B and D genes are presently under investigation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0065-2571
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
159-71
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
A genetic analysis of processes regulating cytochrome P4501A1 expression.
pubmed:affiliation
Laboratory of Structural Biology and Molecular Medicine, University of California, Los Angeles 90024.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't